FOXE1 Antibody (D-6): sc-518211
- FOXE1 Antibody (D-6) is a mouse monoclonal IgG3 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 141-164 of FOXE1 of human origin
- recommended for detection of FOXE1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-FOXE1 Antibody (D-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG3 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for FOXE1 Antibody (D-6) for WB applications. These reagents are now offered in bundles with FOXE1 Antibody (D-6) (see ordering information below).
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FOXE1 Antibody (D-6) is a mouse monoclonal IgG3 kappa light chain antibody that detects FOXE1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). FOXE1 (D-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The forkhead box protein E1 (FOXE1) plays a crucial role in thyroid development and function, as FOXE1 is essential for the differentiation of thyroid follicular cells and the biosynthesis of thyroid hormones. FOXE1 achieves this by regulating the expression of key genes such as the sodium iodide symporter (NIS), thyroid peroxidase (TPO), thyroglobulin (TG), and the thyrotropin receptor (TSHR). FOXE1 is predominantly expressed in several tissues, including the thymus, adult brain, lung, liver, heart, and pancreas, highlighting FOXE1′s importance in various physiological processes. The FOXE1 gene is located on chromosome 9q22, and FOXE1 mutations are associated with congenital hypothyroidism, which affects approximately one in four thousand newborns, leading to significant developmental issues such as thyroid gland underdevelopment. Additionally, individuals with specific mutations in the forkhead domain of the FOXE1 gene may experience thyroid agenesis, cleft palate, and choanal atresia, making FOXE1 a valuable marker for studying these conditions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
FOXE1 Antibody (D-6) References:
- Thyroglobulin regulates follicular function and heterogeneity by suppressing thyroid-specific gene expression. | Suzuki, K., et al. 1999. Biochimie. 81: 329-40. PMID: 10401666
- Cloning, chromosomal localization and identification of polymorphisms in the human thyroid transcription factor 2 gene (TITF2). | Macchia, PE., et al. 1999. Biochimie. 81: 433-40. PMID: 10403172
- Tumor necrosis factor-alpha and interferon-gamma suppress both gene expression and deoxyribonucleic acid-binding of TTF-2 in FRTL-5 cells. | Miyazaki, A., et al. 1999. Endocrinology. 140: 4214-20. PMID: 10465294
- A unique combination of transcription factors controls differentiation of thyroid cells. | Damante, G., et al. 2001. Prog Nucleic Acid Res Mol Biol. 66: 307-56. PMID: 11051768
- Transcriptional activation of the thyroglobulin promoter directing suicide gene expression by thyroid transcription factor-1 in thyroid cancer cells. | Shimura, H., et al. 2001. Cancer Res. 61: 3640-6. PMID: 11325833
- FOXE1 polymorphisms: a new piece in the puzzle of thyroid dysgenesis. | Macchia, PE. 2007. J Endocrinol Invest. 30: 1-2. PMID: 17318014
- TTF-2/FOXE1 gene polymorphisms in Sicilian patients with permanent primary congenital hypothyroidism. | Santarpia, L., et al. 2007. J Endocrinol Invest. 30: 13-9. PMID: 17318017
- FKHL15, a new human member of the forkhead gene family located on chromosome 9q22. | Chadwick, BP., et al. 1997. Genomics. 41: 390-6. PMID: 9169137
- Mutation of the gene encoding human TTF-2 associated with thyroid agenesis, cleft palate and choanal atresia. | Clifton-Bligh, RJ., et al. 1998. Nat Genet. 19: 399-401. PMID: 9697705
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FOXE1 Antibody (D-6) | sc-518211 | 200 µg/ml | $322.00 | |||
Antibody (D-6): m-IgGκ BP-HRP Bundle | sc-551187 | 200 µg Ab; 40 µg BP | $361.00 | |||
Antibody (D-6): m-IgG3 BP-HRP Bundle | sc-551188 | 200 µg Ab; 40 µg BP | $361.00 | |||
FOXE1 Antibody (D-6) AC | sc-518211 AC | 500 µg/ml, 25% agarose | $424.00 | |||
FOXE1 Antibody (D-6) HRP | sc-518211 HRP | 200 µg/ml | $322.00 | |||
FOXE1 Antibody (D-6) FITC | sc-518211 FITC | 200 µg/ml | $336.00 | |||
FOXE1 Antibody (D-6) PE | sc-518211 PE | 200 µg/ml | $349.00 | |||
FOXE1 Antibody (D-6) Alexa Fluor® 488 | sc-518211 AF488 | 200 µg/ml | $364.00 | |||
FOXE1 Antibody (D-6) Alexa Fluor® 546 | sc-518211 AF546 | 200 µg/ml | $364.00 | |||
FOXE1 Antibody (D-6) Alexa Fluor® 594 | sc-518211 AF594 | 200 µg/ml | $364.00 | |||
FOXE1 Antibody (D-6) Alexa Fluor® 647 | sc-518211 AF647 | 200 µg/ml | $364.00 | |||
FOXE1 Antibody (D-6) Alexa Fluor® 680 | sc-518211 AF680 | 200 µg/ml | $364.00 | |||
FOXE1 Antibody (D-6) Alexa Fluor® 790 | sc-518211 AF790 | 200 µg/ml | $364.00 |