FOXD1 Double Nickase Plasmid (h): sc-402521-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FOXD1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FOXD1 Double Nickase Plasmid (h) and FOXD1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FOXD1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FOXD1 Antibody (2C10): sc-293238
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FOXD1 Double Nickase Plasmid (h) | sc-402521-NIC | 20 µg | $410.00 | |||
FOXD1 Double Nickase Plasmid (h2) | sc-402521-NIC-2 | 20 µg | $410.00 |
FOXD1 encodes a forkhead box transcription factor that regulates context-dependent gene expression programs controlling cell fate specification, differentiation, and tissue patterning. In human development, FOXD1 is particularly linked to mesenchymal lineages and kidney morphogenesis, influencing transcriptional networks that coordinate stromal–epithelial signaling, extracellular matrix organization, and organogenesis. Dysregulated FOXD1 expression has been associated with altered lineage commitment and remodeling pathways that intersect with cell-cycle control and developmental signaling cascades. As a nuclear DNA-binding protein, FOXD1 serves as a useful node for dissecting transcriptional regulation, enhancer activity, and developmental pathway cross-talk in disease-relevant cellular models.
FOXD1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FOXD1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FOXD1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FOXD1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FOXD1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.