Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FOXC2 Double Nickase Plasmid (h): sc-402351-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FOXC2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FOXC2 Double Nickase Plasmid (h) and FOXC2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FOXC2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FOXC2 Antibody (G-7): sc-515234
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FOXC2 Double Nickase Plasmid (h) | sc-402351-NIC | 20 µg | $410.00 | |||
FOXC2 Double Nickase Plasmid (h2) | sc-402351-NIC-2 | 20 µg | $410.00 |
FOXC2 encodes a forkhead box transcription factor that regulates lineage specification, epithelial–mesenchymal transition programs, and vascular and lymphatic development through context-dependent control of gene expression. It integrates signaling inputs from pathways such as TGF-β, WNT/β-catenin, and NOTCH to modulate cell migration, extracellular matrix remodeling, and angiogenic/lymphangiogenic responses. Dysregulated FOXC2 activity has been linked to altered lymphatic patterning and developmental phenotypes, and elevated expression is frequently associated with invasive cellular states in cancer models. As a nuclear DNA-binding regulator, FOXC2 is widely studied for its roles in transcriptional networks governing metastasis-associated plasticity and tissue homeostasis.
FOXC2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FOXC2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FOXC2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FOXC2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FOXC2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.