FOXC1 Double Nickase Plasmid (h): sc-403113-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FOXC1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FOXC1 Double Nickase Plasmid (h) and FOXC1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FOXC1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FOXC1 Antibody (4D11): sc-293455
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FOXC1 Double Nickase Plasmid (h) | sc-403113-NIC | 20 µg | $410.00 | |||
FOXC1 Double Nickase Plasmid (h2) | sc-403113-NIC-2 | 20 µg | $410.00 |
FOXC1 (forkhead box C1) is a winged-helix transcription factor that regulates gene expression programs controlling embryonic development, cell fate specification, and tissue patterning, with prominent roles in ocular anterior segment formation, craniofacial morphogenesis, and vascular biology. Through sequence-specific DNA binding, FOXC1 coordinates transcriptional networks that intersect with developmental signaling pathways such as TGF-β/BMP and Wnt, shaping lineage decisions and extracellular matrix remodeling. Dysregulated FOXC1 activity has been linked to congenital anterior segment dysgenesis including Axenfeld–Rieger spectrum phenotypes and contributes to aberrant transcriptional states observed in multiple cancers. In cell models, FOXC1 is often studied for its influence on epithelial–mesenchymal transition, stem-like phenotypes, and context-dependent effects on proliferation, migration, and stress responses.
FOXC1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FOXC1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FOXC1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FOXC1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FOXC1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.