Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Fos B CRISPR/Cas9 KO Plasmid (h): sc-400306
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Fos B CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Fos B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Fos B Antibody (F-7): sc-398595
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Fos B CRISPR/Cas9 KO Plasmid (h) | sc-400306 | 20 µg | $397.00 |
Overview
FOSB encodes Fos B, an immediate early transcription factor of the AP-1 complex that dimerizes with JUN family proteins to regulate stimulus-dependent gene expression. Fos B integrates signals from MAPK/ERK, calcium, and cAMP pathways to control programs linked to cell proliferation, differentiation, survival, and synaptic plasticity. Its rapid induction following growth factor exposure, stress, and neuronal activity makes it a useful marker and mediator of activity-dependent transcription. Dysregulated FOSB/AP-1 signaling has been associated with oncogenic transformation and invasion in multiple tissue contexts, as well as neuropsychiatric and neurodegeneration-related transcriptional remodeling.
Fos B CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FOSB gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FOSB together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FOSB open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Fos B protein expression.
This CRISPR knockout system enables efficient generation of FOSB-deficient cell models for investigation of Fos B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FOSB exon(s) critical for Fos B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FOSB genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Fos B CRISPR/Cas9 KO Plasmid (h) and Fos B CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FOSB locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Fos B HDR Plasmid (h) and Fos B HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FOSB homology arms to support homology-directed repair at defined FOSB target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.