Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FOP CRISPR/Cas9 KO Plasmid (h): sc-407061
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FOP CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FOP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FOP Antibody (B-1): sc-374340
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FOP CRISPR/Cas9 KO Plasmid (h) | sc-407061 | 20 µg | $397.00 |
Overview
FGFR1OP encodes FOP, a centrosome- and microtubule-associated protein implicated in organizing the pericentriolar material and supporting microtubule anchoring during mitosis. Through interactions with centrosomal scaffolds and the cytoskeletal network, FOP contributes to processes including cell-cycle progression, spindle dynamics, and intracellular transport. FGFR1OP has been studied in the context of chromosomal rearrangements involving FGFR1, linking it to aberrant signaling and altered hematopoietic cell behavior. Dysregulation of centrosome function and microtubule organization associated with FOP biology is also relevant to genomic instability phenotypes used in cancer and cell-division research models.
FOP CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FGFR1OP gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FGFR1OP together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FGFR1OP open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FOP protein expression.
This CRISPR knockout system enables efficient generation of FGFR1OP-deficient cell models for investigation of FOP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FGFR1OP exon(s) critical for FOP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FGFR1OP genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FOP CRISPR/Cas9 KO Plasmid (h) and FOP CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FGFR1OP locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FOP HDR Plasmid (h) and FOP HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FGFR1OP homology arms to support homology-directed repair at defined FGFR1OP target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.