FOP Antibody (B-1): sc-374340

FOP Antibody (B-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects FOP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-FOP antibody (B-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FOP protein, also known as FGFR1 oncogene partner, is a 399 amino acid protein that plays a crucial role in cellular organization by localizing to the centrosome, where FOP is essential for anchoring microtubules. This localization is vital for maintaining proper cell division and ensuring mitotic spindle stability, which is critical for accurate chromosome segregation during cell division. FOP is expressed ubiquitously, with highest levels found in kidney, heart, muscle, colon, liver, testis, and pancreas, and functions as a homodimer that interacts with proteins such as EB1 and CEP350. Chromosomal aberrations involving FOP have been linked to stem cell myeloproliferative disorder development, a condition characterized by eosinophilia and myeloid hyperplasia that can progress to acute myeloid leukemia. FOP is expressed as multiple isoforms due to alternative splicing, which may contribute to diverse functional roles in cellular processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

FOP Antibody (B-1) References:

1. [The 8p11 myeloproliferative syndrome].  |  Reither, A., et al. 1999. Med Klin (Munich). 94: 207-10. PMID: 10373756
2. 8p12 stem cell myeloproliferative disorder: the FOP-fibroblast growth factor receptor 1 fusion protein of the t(6;8) translocation induces cell survival mediated by mitogen-activated protein kinase and phosphatidylinositol 3-kinase/Akt/mTOR pathways.  |  Guasch, G., et al. 2001. Mol Cell Biol. 21: 8129-42. PMID: 11689702
3. FOP-FGFR1 tyrosine kinase, the product of a t(6;8) translocation, induces a fatal myeloproliferative disease in mice.  |  Guasch, G., et al. 2004. Blood. 103: 309-12. PMID: 12969958
4. A complex of two centrosomal proteins, CAP350 and FOP, cooperates with EB1 in microtubule anchoring.  |  Yan, X., et al. 2006. Mol Biol Cell. 17: 634-44. PMID: 16314388
5. Structure of the N-terminal domain of the FOP (FGFR1OP) protein and implications for its dimerization and centrosomal localization.  |  Mikolajka, A., et al. 2006. J Mol Biol. 359: 863-75. PMID: 16690081
6. A PP2A phosphatase high density interaction network identifies a novel striatin-interacting phosphatase and kinase complex linked to the cerebral cavernous malformation 3 (CCM3) protein.  |  Goudreault, M., et al. 2009. Mol Cell Proteomics. 8: 157-71. PMID: 18782753
7. The t(6;8)(q27;p11) translocation in a stem cell myeloproliferative disorder fuses a novel gene, FOP, to fibroblast growth factor receptor 1.  |  Popovici, C., et al. 1999. Blood. 93: 1381-9. PMID: 9949182

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FOP Antibody (B-1) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-FOP Antibody (B-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FOP Antibody (B-1) Specifications

Alternate Product Name: FOP

Product Clone Name: B-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: FOP

Epitope Species: human

Product Reactivity/Detection: FOP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for FOP Antibody (B-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin