FOG Antibody (A-6): sc-376189

FOG Antibody (A-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects FOG protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-FOG antibody (A-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FOG protein, known as friend of GATA-1, plays a crucial role in regulating gene expression during hematopoiesis and embryonic development by interacting with GATA family transcription factors. FOG proteins contain multiple zinc finger domains that facilitate binding to GATA proteins, forming complexes essential for the maturation of erythroid and megakaryocyte lineages. FOG′s structure enables these interactions, as the arrangement of zinc fingers allows for specific recognition and binding to GATA factors, thereby influencing transcriptional outcomes. FOG-2, a member of the FOG family, exhibits context-dependent regulatory effects on GATA-mediated transcription, highlighting the complexity of these interactions in different cellular environments. Understanding FOG proteins′ functional dynamics is vital for elucidating their roles in developmental biology and potential implications in hematological disorders.

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FOG Antibody (A-6) References:

1. Transcriptional cofactors of the FOG family interact with GATA proteins by means of multiple zinc fingers.  |  Fox, AH., et al. 1999. EMBO J. 18: 2812-22. PMID: 10329627
2. FOG-2, a heart- and brain-enriched cofactor for GATA transcription factors.  |  Lu, JR., et al. 1999. Mol Cell Biol. 19: 4495-502. PMID: 10330188
3. Friend of GATA-1 represses GATA-3-dependent activity in CD4+ T cells.  |  Zhou, M., et al. 2001. J Exp Med. 194: 1461-71. PMID: 11714753
4. GATA-factor dependence of the multitype zinc-finger protein FOG-1 for its essential role in megakaryopoiesis.  |  Chang, AN., et al. 2002. Proc Natl Acad Sci U S A. 99: 9237-42. PMID: 12077323
5. FOG-2 competes with GATA-4 for transcriptional coactivator p300 and represses hypertrophic responses in cardiac myocytes.  |  Hirai, M., et al. 2004. J Biol Chem. 279: 37640-50. PMID: 15220332
6. FOG-1 represses GATA-1-dependent FcepsilonRI beta-chain transcription: transcriptional mechanism of mast-cell-specific gene expression in mice.  |  Maeda, K., et al. 2006. Blood. 108: 262-9. PMID: 16522818
7. Disruption of friend of GATA 2 gene (FOG-2) by a de novo t(8;10) chromosomal translocation is associated with heart defects and gonadal dysgenesis.  |  Finelli, P., et al. 2007. Clin Genet. 71: 195-204. PMID: 17309641
8. Differential context-dependent effects of friend of GATA-1 (FOG-1) on mast-cell development and differentiation.  |  Sugiyama, D., et al. 2008. Blood. 111: 1924-32. PMID: 18063754
9. FOG, a multitype zinc finger protein, acts as a cofactor for transcription factor GATA-1 in erythroid and megakaryocytic differentiation.  |  Tsang, AP., et al. 1997. Cell. 90: 109-19. PMID: 9230307
10. Failure of megakaryopoiesis and arrested erythropoiesis in mice lacking the GATA-1 transcriptional cofactor FOG.  |  Tsang, AP., et al. 1998. Genes Dev. 12: 1176-88. PMID: 9553047
11. FOG-2: A novel GATA-family cofactor related to multitype zinc-finger proteins Friend of GATA-1 and U-shaped.  |  Tevosian, SG., et al. 1999. Proc Natl Acad Sci U S A. 96: 950-5. PMID: 9927674
12. Molecular cloning of FOG-2: a modulator of transcription factor GATA-4 in cardiomyocytes.  |  Svensson, EC., et al. 1999. Proc Natl Acad Sci U S A. 96: 956-61. PMID: 9927675

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FOG Antibody (A-6) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-FOG Antibody (A-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FOG Antibody (A-6) Specifications

Alternate Product Name: FOG

Product Clone Name: A-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 334-549

Epitope Target: FOG

Epitope Species: mouse

Product Reactivity/Detection: FOG

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for FOG Antibody (A-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent