FN3K Antibody (E-9): sc-271503

FN3K Antibody (E-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects FN3K protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-FN3K antibody (E-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FN3K protein, also known as fructosamine 3-kinase, plays a crucial role in cellular metabolism by catalyzing the ATP-dependent phosphorylation of fructosamines, which are formed when glucose reacts with amines in a process called glycation. This enzymatic action helps destabilize and ultimately remove potentially harmful fructosamines from proteins, thereby protecting them from the detrimental effects of nonenzymatic glycation. FN3K is predominantly expressed in mammalian tissues and is essential for maintaining protein integrity, particularly in the context of diabetes, where abnormal FN3K expression can contribute to complications. Using anti-FN3K antibody (E-9) in research provides valuable insights into the mechanisms of glycation and its implications in metabolic disorders.

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FN3K Antibody (E-9) References:

1. Tissue distribution and evolution of fructosamine 3-kinase and fructosamine 3-kinase-related protein.  |  Delplanque, J., et al. 2004. J Biol Chem. 279: 46606-13. PMID: 15331600
2. Plant ribulosamine/erythrulosamine 3-kinase, a putative protein-repair enzyme.  |  Fortpied, J., et al. 2005. Biochem J. 388: 795-802. PMID: 15705060
3. Intrinsic toxicity of glucose, due to non-enzymatic glycation, is controlled in-vivo by deglycation systems including: FN3K-mediated deglycation of fructosamines and transglycation of aldosamines.  |  Szwergold, BS. 2005. Med Hypotheses. 65: 337-48. PMID: 15922110
4. Some clues as to the regulation, expression, function, and distribution of fructosamine-3-kinase and fructosamine-3-kinase-related protein.  |  Conner, JR., et al. 2005. Ann N Y Acad Sci. 1043: 824-36. PMID: 16037310
5. Transglycation--a potential new mechanism for deglycation of Schiff's bases.  |  Szwergold, BS., et al. 2005. Ann N Y Acad Sci. 1043: 845-64. PMID: 16037312
6. Variability in erythrocyte fructosamine 3-kinase activity in humans correlates with polymorphisms in the FN3K gene and impacts on haemoglobin glycation at specific sites.  |  Delpierre, G., et al. 2006. Diabetes Metab. 32: 31-9. PMID: 16523184
7. Increased protein glycation in fructosamine 3-kinase-deficient mice.  |  Veiga da-Cunha, M., et al. 2006. Biochem J. 399: 257-64. PMID: 16819943
8. The fructosamine 3-kinase knockout mouse: a tool for testing the glycation hypothesis of intracellular protein damage in diabetes and aging.  |  Monnier, VM. 2006. Biochem J. 399: e11-3. PMID: 16987105
9. Fructosamine 3-kinase and other enzymes involved in protein deglycation.  |  Van Schaftingen, E., et al. 2007. Adv Enzyme Regul. 47: 261-9. PMID: 17337043

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FN3K Antibody (E-9) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-FN3K Antibody (E-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FN3K Antibody (E-9) Specifications

Alternate Product Name: FN3K

Product Clone Name: E-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 53-111

Epitope Target: FN3K

Epitope Species: human

Product Reactivity/Detection: FN3K

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for FN3K Antibody (E-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin