Fn14 Antibody (ITEM-4) recommended for detection of Fn14 of mouse, rat and human origin by WB, IP, IF and FCM

Fn14 Antibody (ITEM-4): sc-56250

Fn14 Antibody (ITEM-4) is rated 4.3 out of 5 by 8.
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Datasheets

    • Anti-Fn14 Antibody (ITEM-4) is a mouse monoclonal IgG2b (kappa light chain) Fn14 antibody provided at 200 µg/ml
    • raised against Fn14 of human origin
    • Anti-Fn14 Antibody (ITEM-4) is recommended for detection of Fn14 of mouse, rat and human origin by WB, IP, IF and FCM
    • Anti-Fn14 Antibody (ITEM-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Fn14 (ITEM-4): sc-56250.

Can Fn14 (ITEM-4): sc-56250 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Fn14 (ITEM-4): sc-56250 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Skeletal muscle immunostaining Stained plasma membranes of skeletal muscle fibers.
Date published: 2017-11-22
Rated 5 out of 5 by from Great features! Unique for immunofluoresce I bought this antibody a week ago and it is unique for immunofluoresce. Thanks very much!
Date published: 2017-02-08
Rated 5 out of 5 by from Well established Fn14 antibody Being featured in half a dozen publications in the last few years with beautiful immunoperoxidase staining of formalin fixed, paraffin-embedded human samples, makes for a well characterized monoclonal antibody.
Date published: 2017-01-26
Rated 4 out of 5 by from IHC with paraffin embedded samples (normal IHC with paraffin embedded samples (normal epidermis, hair follicles, and sebaceous and sweat glands ,epidermis from basal cell carcinoma, squamous cell carcinoma and psoriasis). -SCBT Publication Review
Date published: 2015-06-10
Rated 4 out of 5 by from WB: clean band observed in HISM whole cell lysate WB: clean band observed in HISM whole cell lysate. -SCBT QC
Date published: 2015-05-09
Rated 3 out of 5 by from Tried in mouse cells Tried in mouse cells, faint staining in IF, not sure if it is samples or antibody.
Date published: 2015-05-05
Rated 4 out of 5 by from 1:50 for immunoperoxidase staining of paraffin 1:50 for immunoperoxidase staining of paraffin embedded human adipose tissues. -SCBT Publication Review
Date published: 2015-01-02
Rated 4 out of 5 by from Published IHC data using paraffin embedded Published IHC data using paraffin embedded human nephrectomy samples. -SCBT Publication Review
Date published: 2014-09-16
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