Flt-3/Flk-2 Antibody (SF1.340): sc-19635

Flt-3/Flk-2 Antibody (SF1.340) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Flt-3/Flk-2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Flt-3/Flk-2 (SF1.340) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Flt-3/Flk-2, also known as stem cell tyrosine kinase (STK-1), plays a crucial role in hematopoiesis as Flt-3/Flk-2 is involved in the regulation of hematopoietic stem and progenitor cell proliferation and differentiation. Flt-3/Flk-2 is primarily expressed in CD34+ cells, which are essential for the formation of blood cells, and Flt-3/Flk-2 signaling is vital for maintaining the balance between self-renewal and differentiation of these stem cells. The interaction of Flt-3/Flk-2 with its ligands can lead to the activation of downstream signaling pathways that promote cell survival and proliferation, making Flt-3/Flk-2 a significant target for therapeutic interventions in hematological malignancies. The mouse homolog of STK-1, Flt-3/Flk-2, is expressed at high levels in various tissues, including hematopoietic, neural, gonadal, hepatic, and placental tissues, underscoring Flt-3/Flk-2′s importance in both development and immune responses.

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Flt-3/Flk-2 Antibody (SF1.340) References:

1. Expression change of Flk-2/Flt-3 on murine hematopoietic stem cells in an activating state.  |  Hasumura, M., et al. 2003. Exp Hematol. 31: 1331-7. PMID: 14662342
2. A receptor tyrosine kinase specific to hematopoietic stem and progenitor cell-enriched populations.  |  Matthews, W., et al. 1991. Cell. 65: 1143-52. PMID: 1648448
3. Murine Flt3, a gene encoding a novel tyrosine kinase receptor of the PDGFR/CSF1R family.  |  Rosnet, O., et al. 1991. Oncogene. 6: 1641-50. PMID: 1656368
4. Isolation and chromosomal localization of a novel FMS-like tyrosine kinase gene.  |  Rosnet, O., et al. 1991. Genomics. 9: 380-5. PMID: 2004790
5. Serum concentrations of Flt-3 ligand in rheumatic diseases.  |  Nakamura, K., et al. 2015. Biosci Trends. 9: 342-9. PMID: 26559027
6. Prevalence Of FLT-3 Mutation In Acute Myeloid Leukaemia.  |  Sabir, K., et al. 2021. J Ayub Med Coll Abbottabad. 33: 399-402. PMID: 34487645
7. Treatment of relapsed or refractory FLT-3 acute myelogenous leukemia with a triplet regimen of hypomethylating agent, venetoclax, and gilteritinib.  |  Ngo, D., et al. 2024. Leuk Lymphoma. 65: 372-377. PMID: 38164785
8. Concurrent versus sequential or no triazole anti-fungal therapy in patients undergoing 7 + 3 plus midostaurin induction for FLT-3 acute myelogenous leukemia.  |  Ngo, D., et al. 2024. J Oncol Pharm Pract. 10781552241276547. PMID: 39150342
9. STK-1, the human homolog of Flk-2/Flt-3, is selectively expressed in CD34+ human bone marrow cells and is involved in the proliferation of early progenitor/stem cells.  |  Small, D., et al. 1994. Proc Natl Acad Sci U S A. 91: 459-63. PMID: 7507245
10. FLK-2/FLT-3 ligand regulates the growth of early myeloid progenitors isolated from human fetal liver.  |  Muench, MO., et al. 1995. Blood. 85: 963-72. PMID: 7531516
11. Characterization of the protein encoded by the flt3 (flk2) receptor-like tyrosine kinase gene.  |  Lyman, SD., et al. 1993. Oncogene. 8: 815-22. PMID: 7681159
12. Biochemical characterization and analysis of the transforming potential of the FLT3/FLK2 receptor tyrosine kinase.  |  Maroc, N., et al. 1993. Oncogene. 8: 909-18. PMID: 8384358

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Flt-3/Flk-2 Antibody (SF1.340) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-Flt-3/Flk-2 Antibody (SF1.340) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Flt-3/Flk-2 Antibody (SF1.340) Specifications

Alternate Product Name: FLT3

Product Clone Name: SF1.340

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Flt-3/Flk-2

Epitope Species: human

Product Reactivity/Detection: Flt-3/Flk-2 p160 and p130

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for Flt-3/Flk-2 Antibody (SF1.340): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin