FLRG CRISPR/Cas9 KO Plasmid (h): sc-406439
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FLRG CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FLRG genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FLRG CRISPR/Cas9 KO Plasmid (h) | sc-406439 | 20 µg | $397.00 |
Overview
FSTL3 encodes follistatin-like 3 (FLRG), a secreted glycoprotein in the TGF-β superfamily regulatory network that binds and neutralizes activins and related ligands. By modulating activin/TGF-β signaling strength, FLRG influences SMAD-dependent transcriptional programs that control cell proliferation, differentiation, inflammation, and tissue remodeling. Altered FSTL3 expression has been linked to dysregulated endocrine and metabolic homeostasis and to tumor-associated processes such as invasive behavior and stromal interactions. FLRG is therefore a useful node for dissecting extracellular control of growth factor signaling and context-dependent cellular phenotypes.
FLRG CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FSTL3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FSTL3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FSTL3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FLRG protein expression.
This CRISPR knockout system enables efficient generation of FSTL3-deficient cell models for investigation of FLRG signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FSTL3 exon(s) critical for FLRG function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FSTL3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FLRG CRISPR/Cas9 KO Plasmid (h) and FLRG CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FSTL3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FLRG HDR Plasmid (h) and FLRG HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FSTL3 homology arms to support homology-directed repair at defined FSTL3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.