Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Flotillin-1 CRISPR/Cas9 KO Plasmid (m): sc-420381
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Flotillin-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Flotillin-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Flotillin-1 Antibody (C-2): sc-74566
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Flotillin-1 CRISPR/Cas9 KO Plasmid (m) | sc-420381 | 20 µg | $397.00 |
Overview
Flot1 encodes flotillin-1, a conserved lipid raft–associated scaffolding protein enriched at the plasma membrane and endosomal compartments where it helps organize microdomains for signal transduction. Flotillin-1 participates in clathrin-independent endocytosis, membrane trafficking, and cytoskeletal remodeling, influencing receptor internalization and downstream signaling pathways including MAPK/ERK and Src-family kinase networks. In mouse cells, flotillin-1 has been linked to regulation of cell adhesion and migration, immune receptor signaling, and neuronal function through its roles in membrane organization and vesicle dynamics. Dysregulated flotillin biology has been associated with altered proliferative and migratory phenotypes and has been studied in the context of cancer-related signaling, neurodegeneration, and inflammatory processes as a mechanistic modulator rather than a direct effector.
Flotillin-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Flot1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Flot1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Flot1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Flotillin-1 protein expression.
This CRISPR knockout system enables efficient generation of Flot1-deficient cell models for investigation of Flotillin-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Flot1 exon(s) critical for Flotillin-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Flot1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Flotillin-1 CRISPR/Cas9 KO Plasmid (m) and Flotillin-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Flot1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Flotillin-1 HDR Plasmid (m) and Flotillin-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Flot1 homology arms to support homology-directed repair at defined Flot1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.