Flotillin-1 Antibody (F-3): sc-74567

Flotillin-1 Antibody (F-3) is a mouse monoclonal IgG1 antibody that detects Flotillin-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Flotillin-1 plays a crucial role in organizing and functioning of lipid rafts, which are specialized membrane microdomains rich in sphingolipids and cholesterol, essential for various cellular processes including signal transduction and membrane trafficking. Flotillin-1 is primarily localized in the plasma membrane, where Flotillin-1 contributes to forming lipid rafts that facilitate clustering of signaling molecules, thereby enhancing cellular communication efficiency. Flotillin-1 is part of the band 7.2/stomatin protein family and is characterized by a hydrophobic N-terminal region that forms a transmembrane domain, allowing anchoring within the lipid bilayer. Flotillin-1 expression is particularly notable in A498 kidney cells, muscle cell lines, and fibroblasts, and forms a ternary complex with CAP and Cbl, which is vital for directing localization of signaling complexes to lipid raft subdomains. Additionally, Flotillin-1 interaction with other proteins, such as ER-X, is significant for rapid activation of MAP kinase cascade by estrogen, highlighting importance in cellular signaling pathways. Flotillin-1 expression levels are independently regulated and have been correlated with progression of Alzheimer′s pathology, making Flotillin-1 a valuable target for research in neurodegenerative diseases.

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Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.

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Flotillin-1 Antibody (F-3) References:

1. Flotillin-1 stabilizes caveolin-1 in intestinal epithelial cells.  |  Vassilieva, EV., et al. 2009. Biochem Biophys Res Commun. 379: 460-5. PMID: 19121286
2. Flotillin-1/reggie-2 protein plays dual role in activation of receptor-tyrosine kinase/mitogen-activated protein kinase signaling.  |  Amaddii, M., et al. 2012. J Biol Chem. 287: 7265-78. PMID: 22232557
3. Flotillin-1 promotes cell growth and metastasis in oral squamous cell carcinoma.  |  Xiong, P., et al. 2013. Neoplasma. 60: 395-405. PMID: 23581411
4. Flotillin-1 mediates PrPc endocytosis in the cultured cells during Cu²⁺ stimulation through molecular interaction.  |  Ren, K., et al. 2013. Mol Neurobiol. 48: 631-46. PMID: 23625312
5. Flotillin-1 facilitates toll-like receptor 3 signaling in human endothelial cells.  |  Fork, C., et al. 2014. Basic Res Cardiol. 109: 439. PMID: 25204797
6. Identification of the flotillin-1/2 heterocomplex as a target of autoantibodies in bona fide multiple sclerosis.  |  Hahn, S., et al. 2017. J Neuroinflammation. 14: 123. PMID: 28645295
7. Prognostic value of Flotillin-1 expression in patients with solid tumors.  |  Ou, YX., et al. 2017. Oncotarget. 8: 52665-52677. PMID: 28881760
8. Flotillin-1 promotes progression and dampens chemosensitivity to cisplatin in gastric cancer via ERK and AKT signaling pathways.  |  Wei, J., et al. 2022. Eur J Pharmacol. 916: 174631. PMID: 34774850
9. Flotillin-1 is a prognostic biomarker for glioblastoma and promotes cancer development through enhancing invasion and altering tumour microenvironment.  |  Wang, R., et al. 2023. J Cell Mol Med. 27: 392-402. PMID: 36647700
10. Lipid raft protein flotillin-1 is important for the interaction between SOS1 and H-Ras/K-Ras, leading to Ras activation.  |  Jin, H., et al. 2023. Int J Cancer. 152: 1933-1946. PMID: 36691829

The preferred Flotillin-1 antibody is Flotillin-1 Antibody (C-2): sc-74566.