FKBP25 Antibody (H-6): sc-374357
- FKBP25 Antibody (H-6) is a mouse monoclonal IgG2b κ FKBP25 antibody provided at 200 µg/ml
- raised against amino acids 1-170 mapping at the N-terminus of FKBP25 of human origin
- FKBP25 Antibody (H-6) is recommended for detection of FKBP25 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-FKBP25 Antibody (H-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP is the preferred secondary detection reagent for FKBP25 Antibody (H-6) for WB and IHC(P) applications. This reagent is now offered in a bundle with FKBP25 Antibody (H-6) (see ordering information below).
FKBP25 Antibody (H-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects FKBP25 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). FKBP25 (H-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FKBP25, also known as FK506 binding protein 25 or PPIase, is a crucial member of the immunophilin protein family, which plays a significant role in cellular processes by acting as a peptidyl-prolyl isomerase. FKBP25 is primarily localized in the nucleus and is expressed in key tissues such as the brain, testis, ovary, and spleen, where FKBP25 is involved in immunoregulation and the development of the cerebellum. FKBP25 is known to interact with transcriptional repressor protein YY1 and histone deacetylases HDAC1 and HDAC2, which are essential for regulating gene expression. Additionally, FKBP25 may undergo post-translational modifications, including phosphorylation by casein kinase II, which is believed to be critical for regulating cell growth and proliferation. FKBP25′s ability to modulate these pathways highlights its importance in maintaining cellular homeostasis and potential implications in various diseases, making anti-FKBP25 antibody (H-6) a valuable tool for researchers studying these processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
FKBP25 Antibody (H-6) References:
- Rapamycin inhibits didemnin B-induced apoptosis in human HL-60 cells: evidence for the possible involvement of FK506-binding protein 25. | Johnson, KL. and Lawen, A. 1999. Immunol Cell Biol. 77: 242-8. PMID: 10361256
- Down-regulation of the stathmin/Op18 and FKBP25 genes following p53 induction. | Ahn, J., et al. 1999. Oncogene. 18: 5954-8. PMID: 10557083
- The FK506-binding protein 25 functionally associates with histone deacetylases and with transcription factor YY1. | Yang, WM., et al. 2001. EMBO J. 20: 4814-25. PMID: 11532945
- Cloning and identification of a novel cDNA which may be associated with FKBP25. | Meng, X., et al. 2002. Biochem Genet. 40: 303-10. PMID: 12392168
- cDNA cloning of a human 25 kDa FK506 and rapamycin binding protein. | Hung, DT. and Schreiber, SL. 1992. Biochem Biophys Res Commun. 184: 733-8. PMID: 1374240
- Molecular cloning and expression pattern of the Fkbp25 gene during cerebral cortical neurogenesis. | Mas, C., et al. 2005. Gene Expr Patterns. 5: 577-85. PMID: 15908283
- FKBP25 and FKBP38 regulate non-capacitative calcium entry through TRPC6. | Lopez, E., et al. 2015. Biochim Biophys Acta. 1853: 2684-96. PMID: 26239116
- FKBP25 participates in DNA double-strand break repair. | Dilworth, D., et al. 2020. Biochem Cell Biol. 98: 42-49. PMID: 30620620
- Esterase D stabilizes FKBP25 to suppress mTORC1. | Yang, Y., et al. 2021. Cell Mol Biol Lett. 26: 50. PMID: 34875997
- Molecular cloning, DNA sequence analysis, and biochemical characterization of a novel 65-kDa FK506-binding protein (FKBP65). | Coss, MC., et al. 1995. J Biol Chem. 270: 29336-41. PMID: 7493967
- The 25-kDa FK506-binding protein is localized in the nucleus and associates with casein kinase II and nucleolin. | Jin, YJ. and Burakoff, SJ. 1993. Proc Natl Acad Sci U S A. 90: 7769-73. PMID: 7689229
- Cloning and high expression of rabbit FKBP25 in cornea. | Kitagawa, H., et al. 1996. Jpn J Ophthalmol. 40: 133-41. PMID: 8876379
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FKBP25 Antibody (H-6) | sc-374357 | 200 µg/ml | $316.00 | |||
FKBP25 Antibody (H-6): m-IgG2b BP-HRP Bundle | sc-548739 | 200 µg Ab; 10 µg BP | $354.00 | |||
FKBP25 Antibody (H-6) AC | sc-374357 AC | 500 µg/ml, 25% agarose | $416.00 | |||
FKBP25 Antibody (H-6) HRP | sc-374357 HRP | 200 µg/ml | $316.00 | |||
FKBP25 Antibody (H-6) FITC | sc-374357 FITC | 200 µg/ml | $330.00 | |||
FKBP25 Antibody (H-6) PE | sc-374357 PE | 200 µg/ml | $343.00 | |||
FKBP25 Antibody (H-6) Alexa Fluor® 488 | sc-374357 AF488 | 200 µg/ml | $357.00 | |||
FKBP25 Antibody (H-6) Alexa Fluor® 546 | sc-374357 AF546 | 200 µg/ml | $357.00 | |||
FKBP25 Antibody (H-6) Alexa Fluor® 594 | sc-374357 AF594 | 200 µg/ml | $357.00 | |||
FKBP25 Antibody (H-6) Alexa Fluor® 647 | sc-374357 AF647 | 200 µg/ml | $357.00 | |||
FKBP25 Antibody (H-6) Alexa Fluor® 680 | sc-374357 AF680 | 200 µg/ml | $357.00 | |||
FKBP25 Antibody (H-6) Alexa Fluor® 790 | sc-374357 AF790 | 200 µg/ml | $357.00 |