Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FKBP2 Double Nickase Plasmid (h): sc-409613-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FKBP2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FKBP2 Double Nickase Plasmid (h) and FKBP2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FKBP2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FKBP2 Antibody (D-9): sc-390753
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FKBP2 Double Nickase Plasmid (h) | sc-409613-NIC | 20 µg | $410.00 | |||
FKBP2 Double Nickase Plasmid (h2) | sc-409613-NIC-2 | 20 µg | $410.00 |
FKBP2 encodes FK506-binding protein 2, an endoplasmic reticulum–resident peptidyl-prolyl cis–trans isomerase that functions as a molecular chaperone to support folding and quality control of secretory and membrane proteins. As a member of the immunophilin family, FKBP2 contributes to ER proteostasis and integrates with unfolded protein response signaling and ER-associated degradation pathways during cellular stress. Perturbation of ER chaperone capacity and immunophilin-regulated folding dynamics is relevant to mechanisms implicated in protein misfolding disorders and stress-adaptive phenotypes in human cells. FKBP2 is therefore frequently studied in the context of secretory pathway regulation, redox and calcium-linked ER homeostasis, and modulation of protein maturation.
FKBP2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FKBP2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FKBP2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FKBP2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FKBP2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.