FKBP12.6 Antibody (H-8): sc-376135

FKBP12.6 Antibody (H-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects FKBP12.6 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-FKBP12.6 antibody (H-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FKBP12.6, also known as FK506-binding protein 1B, is a 108 amino acid member of the FKBP-type peptidyl-prolyl isomerase family, which plays a crucial role in regulating protein folding and function. FKBP12.6 is primarily located in the cytoplasm, where FKBP12.6 binds to ryanodine receptors in the cardiac muscle sarcoplasmic reticulum, thereby influencing excitation-contraction coupling, a vital process for heart muscle contraction and overall cardiac function. FKBP12.6 is essential for maintaining calcium homeostasis in cardiac cells, and dysregulation has been implicated in various cardiac pathologies. FKBP12.6 is expressed in multiple tissues, with highest levels found in the brain and thymus, and exists in two isoforms due to alternative splicing, highlighting diverse functional roles in different cellular contexts.

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FKBP12.6 Antibody (H-8) References:

1. In situ modulation of the human cardiac ryanodine receptor (hRyR2) by FKBP12.6.  |  George, CH., et al. 2003. Biochem J. 370: 579-89. PMID: 12443530
2. Localization of the 12.6-kDa FK506-binding protein (FKBP12.6) binding site to the NH2-terminal domain of the cardiac Ca2+ release channel (ryanodine receptor).  |  Masumiya, H., et al. 2003. J Biol Chem. 278: 3786-92. PMID: 12446682
3. The binding of the RyR2 calcium channel to its gating protein FKBP12.6 is oppositely affected by ARVD2 and VTSIP mutations.  |  Tiso, N., et al. 2002. Biochem Biophys Res Commun. 299: 594-8. PMID: 12459180
4. Dysregulated ryanodine receptors mediate cellular toxicity: restoration of normal phenotype by FKBP12.6.  |  George, CH., et al. 2003. J Biol Chem. 278: 28856-64. PMID: 12754204
5. Interaction of FKBP12.6 with the cardiac ryanodine receptor C-terminal domain.  |  Zissimopoulos, S. and Lai, FA. 2005. J Biol Chem. 280: 5475-85. PMID: 15591045
6. Genomic organization, chromosomal localization, and promoter of human gene for FK506-binding protein 12.6.  |  Nakazawa, T., et al. 2005. Gene. 360: 55-64. PMID: 16122887
7. Redox sensitivity of the ryanodine receptor interaction with FK506-binding protein.  |  Zissimopoulos, S., et al. 2007. J Biol Chem. 282: 6976-83. PMID: 17200109
8. K201 (JTV519) suppresses spontaneous Ca2+ release and [3H]ryanodine binding to RyR2 irrespective of FKBP12.6 association.  |  Hunt, DJ., et al. 2007. Biochem J. 404: 431-8. PMID: 17313373
9. Removal of FKBP12.6 does not alter the conductance and activation of the cardiac ryanodine receptor or the susceptibility to stress-induced ventricular arrhythmias.  |  Xiao, J., et al. 2007. J Biol Chem. 282: 34828-38. PMID: 17921453
10. FKBP12.6 disruption impairs glucose-induced insulin secretion.  |  Noguchi, N., et al. 2008. Biochem Biophys Res Commun. 371: 735-40. PMID: 18466757
11. FKBP12.6 protects heart from AngII-induced hypertrophy through inhibiting Ca2+ /calmodulin-mediated signalling pathways in vivo and in vitro.  |  Xiao, YF., et al. 2018. J Cell Mol Med. 22: 3638-3651. PMID: 29682889
12. ALK2 Receptor Kinase Association with FKBP12.6 Is Structurally Conserved with the ALK2-FKBP12 Complex.  |  Williams, E., et al. 2021. Biomedicines. 9: PMID: 33572801

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FKBP12.6 Antibody (H-8) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-FKBP12.6 Antibody (H-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FKBP12.6 Antibody (H-8) Specifications

Alternate Product Name: FKBP12.6

Product Clone Name: H-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 38-108

Epitope Target: FKBP12.6

Epitope Species: human

Product Reactivity/Detection: FKBP12.6

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for FKBP12.6 Antibody (H-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin