FKBP11 Double Nickase Plasmid (h): sc-405563-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FKBP11 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FKBP11 Double Nickase Plasmid (h) and FKBP11 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FKBP11. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FKBP11 Antibody (D-3): sc-398700
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FKBP11 Double Nickase Plasmid (h) | sc-405563-NIC | 20 µg | $410.00 | |||
FKBP11 Double Nickase Plasmid (h2) | sc-405563-NIC-2 | 20 µg | $410.00 |
FKBP11 encodes an endoplasmic reticulum–localized FK506-binding protein family member with peptidyl-prolyl cis–trans isomerase activity that supports protein folding and quality control in the secretory pathway. It is enriched in antibody-secreting and other highly secretory cell states, where it contributes to ER proteostasis, folding of nascent polypeptides, and coordination of unfolded protein response signaling. Through these functions, FKBP11 is linked to cellular adaptation to ER stress and regulation of protein maturation and trafficking. Dysregulated ER homeostasis involving FKBP11 has been investigated in contexts of chronic inflammation, immune cell differentiation, and stress-associated pathobiology where secretory load and proteostasis capacity are altered.
FKBP11 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FKBP11 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FKBP11. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FKBP11 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FKBP11-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.