fish Antibody (G-7): sc-376211

fish Antibody (G-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects fish in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-fish antibody (G-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. fish, a potential substrate for Src kinases, is a widely expressed adaptor protein characterized by five SH3 domains and a phox homology (PX) domain, which play crucial roles in cellular signaling and cytoskeletal dynamics. The presence of these SH3 domains allows fish to interact with various signaling molecules, facilitating protein complex assembly essential for signal transduction pathways. Src family protein tyrosine kinases, pivotal in regulating cellular responses to growth factors, can modulate fish activity through tyrosine phosphorylation. This post-translational modification influences protein interactions and functions, especially in cytoskeletal rearrangements and cellular motility. In Src-transformed fibroblasts and normal cells exposed to specific growth factors, fish undergoes tyrosine phosphorylation, indicating involvement in signaling cascades that govern cellular behavior. Additionally, treatment with cytochalasin D rapidly induces tyrosine phosphorylation of fish, further underscoring fish′s role in dynamic regulation of cytoskeletal changes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

fish Antibody (G-7) References:

1. The adaptor protein fish associates with members of the ADAMs family and localizes to podosomes of Src-transformed cells.  |  Abram, CL., et al. 2003. J Biol Chem. 278: 16844-51. PMID: 12615925
2. The Src family of tyrosine protein kinases in hemopoietic signal transduction.  |  Bolen, JB., et al. 1992. FASEB J. 6: 3403-9. PMID: 1281458
3. Newest findings on the oldest oncogene; how activated src does it.  |  Frame, MC. 2004. J Cell Sci. 117: 989-98. PMID: 14996930
4. Discrimination of initiating and promoting carcinogens in fish.  |  Anders, A., et al. 1991. Ann Rech Vet. 22: 273-94. PMID: 1952762
5. Redox and Src family kinase signaling control leukocyte wound attraction and neutrophil reverse migration.  |  Tauzin, S., et al. 2014. J Cell Biol. 207: 589-98. PMID: 25488917
6. Structure-function relationships in Src family and related protein tyrosine kinases.  |  Superti-Furga, G. and Courtneidge, SA. 1995. Bioessays. 17: 321-30. PMID: 7537961
7. Src family protein tyrosine kinases and cellular signal transduction pathways.  |  Erpel, T. and Courtneidge, SA. 1995. Curr Opin Cell Biol. 7: 176-82. PMID: 7612268
8. Regulation, substrates and functions of src.  |  Brown, MT. and Cooper, JA. 1996. Biochim Biophys Acta. 1287: 121-49. PMID: 8672527
9. Role of c-Src tyrosine kinase in G protein-coupled receptor- and Gbetagamma subunit-mediated activation of mitogen-activated protein kinases.  |  Luttrell, LM., et al. 1996. J Biol Chem. 271: 19443-50. PMID: 8702633
10. A role for Pyk2 and Src in linking G-protein-coupled receptors with MAP kinase activation.  |  Dikic, I., et al. 1996. Nature. 383: 547-50. PMID: 8849729
11. A new method for isolating tyrosine kinase substrates used to identify fish, an SH3 and PX domain-containing protein, and Src substrate.  |  Lock, P., et al. 1998. EMBO J. 17: 4346-57. PMID: 9687503

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-fish Antibody (G-7) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-fish Antibody (G-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

fish Antibody (G-7) Specifications

Alternate Product Name: SH3PXD2A

Product Clone Name: G-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 825-1124

Epitope Target: fish

Epitope Species: mouse

Product Reactivity/Detection: fish

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for fish Antibody (G-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin