Filensin Antibody (IB38): sc-69689
- Filensin Antibody (IB38) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against lens filament of bovine origin
- Anti-Filensin Antibody (IB38) is recommended for detection of Filensin of mouse, rat and human origin by WB, IP and IF
- Anti-Filensin Antibody (IB38) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Filensin Antibody (IB38) for WB applications. These reagents are now offered in bundles with Filensin Antibody (IB38) (see ordering information below).
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Filensin Antibody (IB38) is a mouse monoclonal IgG1 kappa light chain antibody that detects Filensin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-Filensin antibody (IB38) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Filensin, also known as CP94, CP95, or CP97, is a critical cytoskeletal intermediate filament protein located in the eye lens fiber, where Filensin plays an essential role in the assembly of beaded filaments that maintain optical clarity. The interaction of Filensin with phakinin is vital for the formation of these filamentous structures, which are crucial for lens development and the regulation of lens fiber cell shape and transparency. Notably, Filensin features a C-terminal non-α-helical domain that is integral to functionality, while the head domain contains a di-arginine/aromatic amino acid motif that includes a potential phosphorylation site for protein kinase A, highlighting the importance of post-translational modifications in modulating activity and interactions within the lens environment.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Filensin Antibody (IB38) References:
- Localization of two conserved cis -acting enhancer regions for the filensin gene promoter that direct lens-specific expression. | Masaki, S., et al. 2002. Exp Eye Res. 75: 295-305. PMID: 12384092
- Tropomodulin binds to filensin intermediate filaments. | Fischer, RS., et al. 2003. FEBS Lett. 547: 228-32. PMID: 12860419
- Targeted deletion of the lens fiber cell-specific intermediate filament protein filensin. | Alizadeh, A., et al. 2003. Invest Ophthalmol Vis Sci. 44: 5252-8. PMID: 14638724
- Filensin is proteolytically processed during lens fiber cell differentiation by multiple independent pathways. | Sandilands, A., et al. 1995. Eur J Cell Biol. 67: 238-53. PMID: 7588880
- Filensin and phakinin form a novel type of beaded intermediate filaments and coassemble de novo in cultured cells. | Goulielmos, G., et al. 1996. J Cell Biol. 132: 643-55. PMID: 8647895
- The mouse filensin gene: structure and evolutionary relation to other intermediate filament genes. | Gounari, F., et al. 1997. FEBS Lett. 413: 371-8. PMID: 9280315
- Gene structure and sequence comparisons of the eye lens specific protein, filensin, from rat and mouse: implications for protein classification and assembly. | Masaki, S. and Quinlan, RA. 1997. Gene. 201: 11-20. PMID: 9409766
- Primary sequence, secondary structure, gene structure, and assembly properties suggests that the lens-specific cytoskeletal protein filensin represents a novel class of intermediate filament protein. | Hess, JF., et al. 1998. Exp Eye Res. 66: 625-44. PMID: 9628810
- Identification and functional analysis of the mouse lens filensin gene promoter. | Masaki, S., et al. 1998. Gene. 214: 77-86. PMID: 9651486
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Filensin Antibody (IB38) | sc-69689 | 200 µg/ml | $322.00 | |||
Filensin Antibody (IB38): m-IgG Fc BP-HRP Bundle | sc-526980 | 200 µg Ab; 10 µg BP | $361.00 | |||
Filensin Antibody (IB38): m-IgG1 BP-HRP Bundle | sc-532353 | 200 µg Ab; 20 µg BP | $361.00 | |||
Filensin Antibody (IB38) AC | sc-69689 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Filensin Antibody (IB38) HRP | sc-69689 HRP | 200 µg/ml | $322.00 | |||
Filensin Antibody (IB38) FITC | sc-69689 FITC | 200 µg/ml | $336.00 | |||
Filensin Antibody (IB38) PE | sc-69689 PE | 200 µg/ml | $349.00 | |||
Filensin Antibody (IB38) Alexa Fluor® 488 | sc-69689 AF488 | 200 µg/ml | $364.00 | |||
Filensin Antibody (IB38) Alexa Fluor® 546 | sc-69689 AF546 | 200 µg/ml | $364.00 | |||
Filensin Antibody (IB38) Alexa Fluor® 594 | sc-69689 AF594 | 200 µg/ml | $364.00 | |||
Filensin Antibody (IB38) Alexa Fluor® 647 | sc-69689 AF647 | 200 µg/ml | $364.00 | |||
Filensin Antibody (IB38) Alexa Fluor® 680 | sc-69689 AF680 | 200 µg/ml | $364.00 | |||
Filensin Antibody (IB38) Alexa Fluor® 790 | sc-69689 AF790 | 200 µg/ml | $364.00 |