fidgetin Antibody (F-2): sc-514956

Fidgetin Antibody (F-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects fidgetin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-fidgetin antibody (F-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Fidgetin protein, encoded by the mouse Fign gene, consists of 759 amino acids and belongs to the AAA (ATPases associated with diverse cellular activities) family, specifically the meiotic or subfamily-7 group. Fidgetin (F-2) monoclonal antibody recognizes a protein that plays a crucial role in cellular processes such as membrane fusion, proteolysis, and meiotic spindle formation, highlighting its importance in maintaining cellular integrity and function. Fidgetin can self-interact, a property that is essential for its function, and this interaction can be disrupted by truncating either the N- or C-terminus of the protein. The spontaneous mutation known as fidget in mice leads to distinctive behavioral traits, including side-to-side head shaking and circling, which are attributed to the reduced or absent semicircular canals. Additionally, fidget mice exhibit small eyes due to cell-cycle delays and insufficient growth of the retinal neural epithelium, along with lower penetrance skeletal abnormalities such as pelvic girdle dysgenesis, skull bone fusions, and polydactyly, making the study of fidgetin relevant for understanding cellular mechanisms and developmental anomalies.

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fidgetin Antibody (F-2) References:

1. The mouse fidgetin gene defines a new role for AAA family proteins in mammalian development.  |  Cox, GA., et al. 2000. Nat Genet. 26: 198-202. PMID: 11017077
2. Identification of a cysteine residue important for the ATPase activity of C. elegans fidgetin homologue.  |  Yakushiji, Y., et al. 2004. FEBS Lett. 578: 191-7. PMID: 15581640
3. Functional characterization of fidgetin, an AAA-family protein mutated in fidget mice.  |  Yang, Y., et al. 2005. Exp Cell Res. 304: 50-8. PMID: 15707573
4. Mutational analysis of the functional motifs in the ATPase domain of Caenorhabditis elegans fidgetin homologue FIGL-1: firm evidence for an intersubunit catalysis mechanism of ATP hydrolysis by AAA ATPases.  |  Yakushiji, Y., et al. 2006. J Struct Biol. 156: 93-100. PMID: 16621600
5. Interaction between fidgetin and protein kinase A-anchoring protein AKAP95 is critical for palatogenesis in the mouse.  |  Yang, Y., et al. 2006. J Biol Chem. 281: 22352-22359. PMID: 16751186
6. Fidgetin-like 1 gene inhibited by basic fibroblast growth factor regulates the proliferation and differentiation of osteoblasts.  |  Park, SJ., et al. 2007. J Bone Miner Res. 22: 889-96. PMID: 17352653
7. The AAA-ATPase FIGL-1 controls mitotic progression, and its levels are regulated by the CUL-3MEL-26 E3 ligase in the C. elegans germ line.  |  Luke-Glaser, S., et al. 2007. J Cell Sci. 120: 3179-87. PMID: 17878235

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-fidgetin Antibody (F-2) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-fidgetin Antibody (F-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

fidgetin Antibody (F-2) Specifications

Alternate Product Name: fidgetin

Product Clone Name: F-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 75-220

Epitope Target: fidgetin

Epitope Species: human

Product Reactivity/Detection: fidgetin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for fidgetin Antibody (F-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin