Date published: 2025-9-27

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Fibronectin Antibody (C6F10): sc-73611

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Datasheets
  • Fibronectin Antibody (C6F10) is a mouse monoclonal IgG1 κ, cited in 13 publications, provided at 200 µg/ml
  • raised against plasma Fibronectin of human origin
  • Anti-Fibronectin Antibody (C6F10) is recommended for detection of Fibronectin III-10 of human, monkey, porcine, bovine, equine, avian and amphibian origin by WB, IP, IF and IHC(P); non cross-reactive with other serum proteins
  • Anti-Fibronectin Antibody (C6F10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP is the preferred secondary detection reagent for Fibronectin Antibody (C6F10) for WB and IHC(P) applications. This reagent is now offered in a bundle with Fibronectin Antibody (C6F10) (see ordering information below).

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Fibronectin Antibody (C6F10) is a mouse monoclonal IgG1 kappa light chain antibody that detects human Fibronectin through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Fibronectin antibody (C6F10) comes in non-conjugated form and various conjugates, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® options, offering versatility for different experimental needs. Fibronectin is a high molecular weight extracellular matrix glycoprotein essential for cell adhesion, motility, and wound healing, present on most cell surfaces, in extracellular fluids, and plasma. As a heterodimer, Fibronectin undergoes alternative splicing and glycosylation, producing various isoforms with tissue-specific distribution and associations with certain malignancies. Glycosylated Fibronectin and specific splice variants have been connected to myofibroblast phenotype formation in conditions like Dupuytren′s disease, highlighting Fibronectin′s significance in both normal physiology and pathological conditions. Fibronectin (C6F10) monoclonal antibody shows cross-reactivity with monkey, porcine, bovine, equine, avian, and amphibian tissues, making this antibody valuable for diverse biological research applications.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Fibronectin Antibody (C6F10)

sc-73611
200 µg/ml
$316.00

Fibronectin Antibody (C6F10): m-IgG Fc BP-HRP Bundle

sc-525700
200 µg Ab; 10 µg BP
$354.00

Fibronectin Antibody (C6F10) AC

sc-73611 AC
500 µg/ml, 25% agarose
$416.00

Fibronectin Antibody (C6F10) HRP

sc-73611 HRP
200 µg/ml
$316.00

Fibronectin Antibody (C6F10) FITC

sc-73611 FITC
200 µg/ml
$330.00

Fibronectin Antibody (C6F10) PE

sc-73611 PE
200 µg/ml
$343.00

Fibronectin Antibody (C6F10) Alexa Fluor® 488

sc-73611 AF488
200 µg/ml
$357.00

Fibronectin Antibody (C6F10) Alexa Fluor® 546

sc-73611 AF546
200 µg/ml
$357.00

Fibronectin Antibody (C6F10) Alexa Fluor® 594

sc-73611 AF594
200 µg/ml
$357.00

Fibronectin Antibody (C6F10) Alexa Fluor® 647

sc-73611 AF647
200 µg/ml
$357.00

Fibronectin Antibody (C6F10) Alexa Fluor® 680

sc-73611 AF680
200 µg/ml
$357.00

Fibronectin Antibody (C6F10) Alexa Fluor® 790

sc-73611 AF790
200 µg/ml
$357.00

Hi, I tried this antibody for porcine colon tissue lysate and it did not work. The WB results always show the roughly 50 kDa band, not 230kDa as in the datasheet. Are you sure this antibody is also reactive with porcine fibronectin?

Asked by: danK
Thank you for your question. Please contact technical service directly for troubleshooting advice on your experiment.
Answered by: Tech Support Europe
Date published: 2021-02-03

Does this antibody react with fibronectin of mouse origin, say fibronectin from 3T3? Best regards, Zheng

Asked by: Beer
Thank you for your question. This antibody is not recommended for detection of Fibronectin of mouse origin. We have many alternative Fibronectin antibodies that are recommended for mouse. Please contact our Technical Service Department for help in selecting the best antibody for your research applications.
Answered by: Technical Support
Date published: 2019-02-23

How can Fibronectin (C6F10): sc-73611 monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenniferc
Thank you for your question. In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. Please contact our special orders department for pricing and availability of sc-73611 conjugates or consider using Fibronectin (EP5): sc-8422 , which is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. Please visit this link for more information: https://www.scbt.com/scbt/product/fibronectin-antibody-ep5
Answered by: Santa Cruz TS
Date published: 2017-01-31
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Rated 4 out of 5 by from 良好な結果ウエスタンブロッティングで使用したが、目的の分子量と概ね一致したバンドが検出された。今度は蛍光免疫染色で使用してみたい。
Date published: 2020-07-07
Rated 5 out of 5 by from GreatI used this for flow cytometry both unfixed and fixed. Worked great in both ways.
Date published: 2019-06-19
Rated 2 out of 5 by from temperamentalWorks only sometimes...we have not had much luck with this antibody. We've tried all tricks though.
Date published: 2018-12-22
Rated 4 out of 5 by from Great antibodiesI got a free sample from company. I used antibodies in WB in dilution 1:1000. It works good.
Date published: 2018-07-14
Rated 4 out of 5 by from Good antibodyIt works well to detect purified fibronectin in Western blot at 1:200 dilution
Date published: 2018-03-12
Rated 5 out of 5 by from Highly recommended for IHC(P)Fantastic immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue!
Date published: 2017-01-14
Rated 5 out of 5 by from Good bands with UGood bands with U-87 MG and HT-1080 cell lysates in WB -SCBT QC
Date published: 2014-03-10
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Fibronectin Antibody (C6F10) is rated 4.1 out of 5 by 7.
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