Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Fibromodulin Double Nickase Plasmid (h): sc-401610-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Fibromodulin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Fibromodulin Double Nickase Plasmid (h) and Fibromodulin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FMOD. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Fibromodulin Antibody (H-11): sc-166406
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Fibromodulin Double Nickase Plasmid (h) | sc-401610-NIC | 20 µg | $410.00 | |||
Fibromodulin Double Nickase Plasmid (h2) | sc-401610-NIC-2 | 20 µg | $410.00 |
Human FMOD encodes fibromodulin, a small leucine-rich proteoglycan of the extracellular matrix that binds collagen fibrils and modulates fibrillogenesis, matrix assembly, and tissue mechanical properties. Fibromodulin participates in connective tissue homeostasis and influences cell–matrix signaling through pathways linked to integrin engagement, TGF-β activity, and wound repair programs. Altered FMOD expression or extracellular matrix remodeling involving fibromodulin has been associated with fibrotic responses and tumor-associated stromal changes, making it relevant to studies of microenvironmental regulation. As an ECM-regulatory factor, FMOD is frequently examined in models of inflammation-driven remodeling, angiogenesis, and mechanotransduction.
Fibromodulin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FMOD locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FMOD. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FMOD function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FMOD-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.