Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Fibrillarin CRISPR/Cas9 KO Plasmid (h): sc-400741
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Fibrillarin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Fibrillarin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Fibrillarin Antibody (G-8): sc-374022
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Fibrillarin CRISPR/Cas9 KO Plasmid (h) | sc-400741 | 20 µg | $397.00 |
Overview
FBL encodes fibrillarin, an essential nucleolar methyltransferase that catalyzes 2′-O-ribose methylation of pre-rRNA within box C/D snoRNP complexes, supporting ribosome biogenesis and translational capacity. Fibrillarin contributes to pre-rRNA processing, nucleolar organization, and coordination of RNA modification with cell growth and stress responses. Altered nucleolar homeostasis and ribosome production are recurrent features of proliferative and neurodevelopmental pathologies, and FBL dysregulation has been linked to changes in RNA processing programs and cellular fitness. Fibrillarin is also a well-characterized autoantigen in systemic autoimmune disease, connecting nucleolar RNP biology to immune recognition.
Fibrillarin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FBL gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FBL together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FBL open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Fibrillarin protein expression.
This CRISPR knockout system enables efficient generation of FBL-deficient cell models for investigation of Fibrillarin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FBL exon(s) critical for Fibrillarin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FBL genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Fibrillarin CRISPR/Cas9 KO Plasmid (h) and Fibrillarin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FBL locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Fibrillarin HDR Plasmid (h) and Fibrillarin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FBL homology arms to support homology-directed repair at defined FBL target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.