Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FHOD1 Double Nickase Plasmid (h): sc-404286-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FHOD1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FHOD1 Double Nickase Plasmid (h) and FHOD1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FHOD1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FHOD1 Antibody (D-6): sc-365437
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FHOD1 Double Nickase Plasmid (h) | sc-404286-NIC | 20 µg | $410.00 | |||
FHOD1 Double Nickase Plasmid (h2) | sc-404286-NIC-2 | 20 µg | $410.00 |
FHOD1 (formin homology 2 domain containing 1) encodes a Diaphanous-related formin that promotes actin filament elongation and organization, supporting stress fiber assembly, cell polarity, and adhesion-dependent migration. As an effector downstream of Rho-family GTPase signaling, FHOD1 integrates cues from cytoskeletal remodeling pathways to regulate contractility and membrane protrusion dynamics. Altered FHOD1 activity has been linked to changes in cell motility and invasive behavior, and dysregulation of actin architecture implicates FHOD1 in studies of tumor progression, cardiovascular and fibrotic remodeling, and neurodevelopmental processes. Its localization and function at actin-rich structures make it a useful node for dissecting mechanotransduction and cytoskeleton–nucleus coupling.
FHOD1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FHOD1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FHOD1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FHOD1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FHOD1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.