FGF-10 Antibody (3C7) recommended for detection of FGF-10 of human origin by WB, IP and ELISA

FGF-10 Antibody (3C7): sc-293208

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Datasheets

    • Anti-FGF-10 Antibody (3C7) is a mouse monoclonal IgG2a (kappa light chain) FGF-10 antibody provided at 100 µg/ml
    • raised against amino acids 38-137 of FGF-10 of human origin
    • recommended for detection of FGF-10 of human origin by WB, IP and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Hello. For sc-293208, was as the immunogen used FGF10 recombinant protein with or without GST tag? I am asking because I found antibody with the same name "FGF10-antibody (C37)" in different company and this antibody was with GST-tag.

Asked by: NikolaPt
Thank you for your question. Unfortunately we do not provide this information, however standard procedure for monoclonal antibody production using a recombinant protein does include GST conjugation (and GST subtraction later in the process).
Answered by: Tech Service
Date published: 2019-09-26

For Western Blot, is it recommended to use denatured or non-denatured conditions with FGF-10 (3C7): sc-293208 antibody?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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