Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Fes Double Nickase Plasmid (h): sc-403246-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Fes Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Fes Double Nickase Plasmid (h) and Fes Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FES. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Fes Antibody (D-9): sc-377179
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Fes Double Nickase Plasmid (h) | sc-403246-NIC | 20 µg | $410.00 | |||
Fes Double Nickase Plasmid (h2) | sc-403246-NIC-2 | 20 µg | $410.00 |
FES encodes Fes, a non-receptor tyrosine kinase that integrates signals from cytokine and growth factor receptors to regulate phosphorylation-dependent programs controlling cell adhesion, migration, and differentiation. Fes participates in pathways linked to cytoskeletal remodeling and vesicular/actin dynamics, influencing myeloid and endothelial cell behavior and cellular responses to inflammatory cues. Dysregulated tyrosine kinase signaling involving Fes has been associated with altered proliferative and survival signaling in hematopoietic lineages and other contexts, making it a useful node for mechanistic studies of signal transduction. In cancer biology and immunology research, FES is often examined for its impact on kinase-driven networks that modulate cellular plasticity and microenvironmental interactions.
Fes Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FES locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FES. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FES function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FES-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.