Fes Antibody (D-9): sc-377179

Fes Antibody (D-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects Fes protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Fes (D-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Fes protein, a member of the cytoplasmic class of protein tyrosine kinases, plays a crucial role in myeloid differentiation and is encoded by the proto-oncogene c-fes. Fes structure includes an SH2 domain, which is essential for mediating protein-protein interactions, and autophosphorylation capabilities that are vital for kinase activity. Fes interaction with various hematopoietic cytokine receptors and the B-cell receptor (BCR) highlights Fes importance in signaling pathways that regulate immune responses. Notably, phosphorylation of BCR by Fes facilitates BCR association with the Ras guanine nucleotide exchange factor complex GRB2/Sos, thereby influencing downstream signaling events critical for B-cell activation and differentiation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Fes Antibody (D-9) References:

1. Fully automated structure determinations of the Fes SH2 domain using different sets of NMR spectra.  |  Scott, A., et al. 2006. Magn Reson Chem. 44 Spec No: S83-8. PMID: 16826546
2. Insulin-like growth factor I receptor gene is concordant with c-Fes protooncogene and mouse chromosome 7 in somatic cell hybrids.  |  Sundaresan, S. and Francke, U. 1989. Somat Cell Mol Genet. 15: 373-6. PMID: 2548293
3. Human cellular fps/fes cDNA rescued via retroviral shuttle vector encodes myeloid cell NCP92 and has transforming potential.  |  Feldman, RA., et al. 1987. Oncogene Res. 1: 441-58. PMID: 3329718
4. Localization of the cellular oncogenes ABL, SIS, and FES on human germ-line chromosomes.  |  Jhanwar, SC., et al. 1984. Cytogenet Cell Genet. 38: 73-5. PMID: 6323103
5. Transforming potential of a human protooncogene (c-fps/fes) locus.  |  Sodroski, JG., et al. 1984. Proc Natl Acad Sci U S A. 81: 3039-43. PMID: 6328490
6. Genetic mapping of the mouse oncogenes c-Ha-ras-1 and c-fes to chromosome 7.  |  Kozak, CA., et al. 1983. J Virol. 47: 217-20. PMID: 6864883
7. Interaction of the c-fes proto-oncogene product with the interleukin-4 receptor.  |  Izuhara, K., et al. 1994. J Biol Chem. 269: 18623-9. PMID: 7518439
8. Tyrosine phosphorylation of BCR by FPS/FES protein-tyrosine kinases induces association of BCR with GRB-2/SOS.  |  Maru, Y., et al. 1995. Mol Cell Biol. 15: 835-42. PMID: 7529874
9. Regulation of the human c-fes protein tyrosine kinase (p93c-fes) by its src homology 2 domain and major autophosphorylation site (Tyr-713).  |  Hjermstad, SJ., et al. 1993. Oncogene. 8: 2283-92. PMID: 7687763
10. Phosphorylation of the ras GTPase-activating protein (GAP) by the p93c-fes protein-tyrosine kinase in vitro and formation of GAP-fes complexes via an SH2 domain-dependent mechanism.  |  Hjermstad, SJ., et al. 1993. Biochemistry. 32: 10519-25. PMID: 7691175
11. Autophosphorylation of the Fes tyrosine kinase. Evidence for an intermolecular mechanism involving two kinase domain tyrosine residues.  |  Rogers, JA., et al. 1996. J Biol Chem. 271: 17519-25. PMID: 8663427
12. The Fes protein-tyrosine kinase phosphorylates a subset of macrophage proteins that are involved in cell adhesion and cell-cell signaling.  |  Jücker, M., et al. 1997. J Biol Chem. 272: 2104-9. PMID: 8999909

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Fes Antibody (D-9) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Fes Antibody (D-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Fes Antibody (D-9) Specifications

Alternate Product Name: Fes

Product Clone Name: D-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 758-822

Epitope Target: Fes

Epitope Species: human

Product Reactivity/Detection: Fes

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Fes Antibody (D-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin