Ferroportin-1 Antibody (B-4): sc-518125

Ferroportin-1 (B-4) is a mouse monoclonal IgM kappa antibody that detects Ferroportin of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF) and enzyme-linked immunosorbent assay (ELISA). Anti-Ferroportin-1 antibody (B-4) is available as non-conjugated form, as well as multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. Ferroportin-1, also designated solute carrier family 40 member 1 or iron-regulated transporter 1, is a multi-pass membrane protein that belongs to the SLC40A transporter protein family and localizes to the cell membrane on various cells, especially hepatocytes, endothelial cells and enterocytes. Ferroportin-1 plays a role in iron transfer between maternal and fetal circulation and acts as a mediator of iron efflux in the presence of ferroxidases, such as ceruloplasmin or hephaestin. Ferroportin-1 may be involved in iron export from duodenal epithelial cells. Ferroportin-1 is expressed at highest levels in intestine, muscle, spleen and placenta. Mutations in SLC40A cause hemochromatosis type 4 (HFE4), an autosomal dominant disorder characterized by excess storage of iron in reticuloendothelial cells and an increase in serum ferritin before elevation of the transferrin saturation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Ferroportin-1 Antibody (B-4) References:

1. Iron release from macrophages after erythrophagocytosis is up-regulated by ferroportin 1 overexpression and down-regulated by hepcidin.  |  Knutson, MD., et al. 2005. Proc Natl Acad Sci U S A. 102: 1324-8. PMID: 15665091
2. Iron transporters in rat mammary gland: effects of different stages of lactation and maternal iron status.  |  Leong, WI. and Lönnerdal, B. 2005. Am J Clin Nutr. 81: 445-53. PMID: 15699234
3. Apical location of ferroportin 1 in airway epithelia and its role in iron detoxification in the lung.  |  Yang, F., et al. 2005. Am J Physiol Lung Cell Mol Physiol. 289: L14-23. PMID: 15749737
4. Ferroportin1 is required for normal iron cycling in zebrafish.  |  Fraenkel, PG., et al. 2005. J Clin Invest. 115: 1532-41. PMID: 15902304
5. Regulation of hepcidin and ferroportin expression by lipopolysaccharide in splenic macrophages.  |  Liu, XB., et al. 2005. Blood Cells Mol Dis. 35: 47-56. PMID: 15932798
6. Functional consequences of ferroportin 1 mutations.  |  Liu, XB., et al. 2005. Blood Cells Mol Dis. 35: 33-46. PMID: 15935710
7. A novel ferroportin mutation in a Canadian family with autosomal dominant hemochromatosis.  |  Morris, TJ., et al. 2005. Blood Cells Mol Dis. 35: 309-14. PMID: 16111902
8. Fasting up-regulates ferroportin 1 expression via a Ghrelin/GHSR/MAPK signaling pathway.  |  Luo, QQ., et al. 2018. J Cell Physiol. 233: 30-37. PMID: 28338217
9. Regulating ferroportin-1 and transferrin receptor-1 expression: A novel function of hydrogen sulfide.  |  Zhang, MW., et al. 2019. J Cell Physiol. 234: 3158-3169. PMID: 30370692
10. Astrocyte-derived hepcidin controls iron traffic at the blood-brain-barrier via regulating ferroportin 1 of microvascular endothelial cells.  |  You, L., et al. 2022. Cell Death Dis. 13: 667. PMID: 35915080
11. Role of hepcidin upregulation and proteolytic cleavage of ferroportin 1 in hepatitis C virus-induced iron accumulation.  |  Ohta, K., et al. 2023. PLoS Pathog. 19: e1011591. PMID: 37585449
12. The mechanism of hypoxia-inducible factor-1α enhancing the transcriptional activity of transferrin ferroportin 1 and regulating the Nrf2/HO-1 pathway in ferroptosis after cerebral ischemic injury.  |  Yao, H., et al. 2024. Neuroscience. 559: 26-38. PMID: 39168172

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ferroportin-1 Antibody (B-4) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Ferroportin-1 Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ferroportin-1 Antibody (B-4) Specifications

Alternate Product Name: Ferroportin-1

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 391-413

Epitope Target: Ferroportin-1

Epitope Species: human

Product Reactivity/Detection: Ferroportin

Species Reactivity/Detection: human

Additional Mammalian Reactivity: bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Ferroportin-1 Antibody (B-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin