Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ferritin light chain CRISPR/Cas9 KO Plasmid (h): sc-400924
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ferritin light chain CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ferritin light chain genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ferritin light chain Antibody (D-9): sc-74513
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ferritin light chain CRISPR/Cas9 KO Plasmid (h) | sc-400924 | 20 µg | $397.00 |
Overview
FTL encodes the ferritin light chain, a core component of the ferritin heteropolymer that sequesters and mineralizes iron to maintain cellular iron homeostasis and limit iron-driven oxidative stress. By buffering labile iron pools, FTL influences redox balance, mitochondrial function, and iron-responsive signaling through the IRP/IRE regulatory network, coordinating iron storage with iron uptake and utilization. Altered ferritin composition or expression can perturb ferroptosis sensitivity and inflammatory stress responses by changing iron availability and lipid peroxidation propensity. Dysregulated FTL and ferritin biology have been linked to neurodegeneration, anemia-associated iron mismanagement, and cancer-related metabolic remodeling, making FTL a useful node for studying iron-dependent cellular phenotypes.
ferritin light chain CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FTL gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FTL together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FTL open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ferritin light chain protein expression.
This CRISPR knockout system enables efficient generation of FTL-deficient cell models for investigation of ferritin light chain signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FTL exon(s) critical for ferritin light chain function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FTL genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ferritin light chain CRISPR/Cas9 KO Plasmid (h) and ferritin light chain CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FTL locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ferritin light chain HDR Plasmid (h) and ferritin light chain HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FTL homology arms to support homology-directed repair at defined FTL target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.