Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ferritin heavy chain Antibody (B-12): sc-376594. Near-infrared western blot analysis of ferritin heavy chain expression in U-87 MG (A), Jurkat (B) and MCF7 (C) whole cell lysates. Blocked with UltraCruz® Blocking Reagent: sc-516214. Detection reagent used: m-IgGκ BP-CFL 790: sc-516181.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ferritin heavy chain CRISPR Activation Plasmid (h): sc-418152-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ferritin heavy chain CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- ferritin heavy chain CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by ferritin heavy chain CRISPR Activation Plasmid (h) and ferritin heavy chain CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the FTH1 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ferritin heavy chain Antibody (B-12): sc-376594
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ferritin heavy chain CRISPR Activation Plasmid (h) | sc-418152-ACT | 20 µg | $397.00 | |||
ferritin heavy chain CRISPR Activation Plasmid (h2) | sc-418152-ACT-2 | 20 µg | $397.00 |