FCP1 Antibody (3G4): sc-293358

FCP1 Antibody (3G4) is a mouse monoclonal IgG2a kappa light chain antibody that detects FCP1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Anti-FCP1 antibody (3G4) is available as the non-conjugated format. FCP1, also known as TFIIF-associated CTD phosphatase, plays a crucial role in regulating RNA polymerase II (RNAP II) during the transcription cycle. Specifically, FCP1 is responsible for dephosphorylating the carboxy terminal domain (CTD) of RNAP II, which is essential for the transition between transcription initiation and elongation. The CTD consists of 52 repeats of a heptapeptide sequence that undergoes phosphorylation at specific serine residues, which is critical for the recruitment of transcription factors and the progression of RNA synthesis. FCP1 selectively targets phosphorylated serine 2 and 5 of the CTD, and FCP1 phosphorylation is necessary for activity and interaction with the transcription factor TFIIF. Upon activation, the carboxy terminus of FCP1 forms a complex with RAP74, further highlighting FCP1′s importance in transcription regulation. Additionally, FCP1 has been shown to repress HIV-1 Tat-mediated transactivation, suggesting FCP1′s potential as a therapeutic target in the modulation of viral activity in infected cells.

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FCP1 Antibody (3G4) References:

1. Inhibition of Tat transactivation by the RNA polymerase II CTD-phosphatase FCP1.  |  Licciardo, P., et al. 2001. AIDS. 15: 301-7. PMID: 11273209
2. Transcription activation by targeted recruitment of the RNA polymerase II CTD phosphatase FCP1.  |  Licciardo, P., et al. 2001. Nucleic Acids Res. 29: 3539-45. PMID: 11522823
3. TFIIF-associating carboxyl-terminal domain phosphatase dephosphorylates phosphoserines 2 and 5 of RNA polymerase II.  |  Lin, PS., et al. 2002. J Biol Chem. 277: 45949-56. PMID: 12351650
4. FCP1, a phosphatase specific for the heptapeptide repeat of the largest subunit of RNA polymerase II, stimulates transcription elongation.  |  Mandal, SS., et al. 2002. Mol Cell Biol. 22: 7543-52. PMID: 12370301
5. The C-terminal domain phosphatase and transcription elongation activities of FCP1 are regulated by phosphorylation.  |  Friedl, EM., et al. 2003. Proc Natl Acad Sci U S A. 100: 2328-33. PMID: 12591939
6. Molecular mechanism of recruitment of TFIIF- associating RNA polymerase C-terminal domain phosphatase (FCP1) by transcription factor IIF.  |  Kamada, K., et al. 2003. Proc Natl Acad Sci U S A. 100: 2296-9. PMID: 12591941

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FCP1 Antibody (3G4) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-FCP1 Antibody (3G4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FCP1 Antibody (3G4) Specifications

Alternate Product Name: FCP1

Product Clone Name: 3G4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 332-431

Epitope Target: FCP1

Epitope Species: human

Product Reactivity/Detection: FCP1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for FCP1 Antibody (3G4): N/A