Fc ε RIγ Double Nickase Plasmid (h): sc-417621-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Fc ε RIγ Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Fc ε RIγ Double Nickase Plasmid (h) and Fc ε RIγ Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FCER1G. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Fc ε RIγ Antibody (E-12): sc-390222
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Fc ε RIγ Double Nickase Plasmid (h) | sc-417621-NIC | 20 µg | $410.00 | |||
Fc ε RIγ Double Nickase Plasmid (h2) | sc-417621-NIC-2 | 20 µg | $410.00 |
FCER1G encodes the FcεRIγ signaling subunit, an ITAM-bearing adaptor that pairs with multiple immune receptors to couple ligand engagement to intracellular activation cascades. In myeloid and mast cell contexts, FcεRIγ participates in Fc receptor and C-type lectin receptor signaling, promoting Src/Syk kinase activation, downstream calcium flux, MAPK signaling, and transcriptional programs that regulate degranulation, cytokine production, and phagocytic responses. Through these pathways, FcεRIγ helps shape innate and antibody-dependent effector functions and is frequently studied in allergic inflammation, infection biology, and immune dysregulation. Altered expression or signaling through FcεRIγ-associated receptor complexes has been linked to inflammatory phenotypes and provides a mechanistic entry point for dissecting immune cell activation states.
Fc ε RIγ Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FCER1G locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FCER1G. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FCER1G function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FCER1G-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.