FBXW2 CRISPR/Cas9 KO Plasmid (h): sc-406409
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FBXW2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FBXW2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FBXW2 CRISPR/Cas9 KO Plasmid (h) | sc-406409 | 20 µg | $397.00 |
Overview
FBXW2 encodes an F-box and WD repeat–containing protein that functions as a substrate recognition component of SCF (SKP1–CUL1–F-box) E3 ubiquitin ligase complexes, helping direct specific proteins for ubiquitination and proteasomal degradation. Through regulated turnover of signaling and cell-cycle–associated factors, FBXW2 contributes to proteostasis and fine-tuning of pathways that control proliferation, differentiation, and stress responses. Altered ubiquitin-mediated regulation is a common feature of oncogenic signaling and other disorders involving disrupted protein homeostasis, making FBXW2 a relevant node for mechanistic studies of pathway rewiring. FBXW2 is therefore of interest for investigating how targeted degradation programs influence cellular phenotype in disease-relevant contexts.
FBXW2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FBXW2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FBXW2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FBXW2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FBXW2 protein expression.
This CRISPR knockout system enables efficient generation of FBXW2-deficient cell models for investigation of FBXW2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FBXW2 exon(s) critical for FBXW2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FBXW2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FBXW2 CRISPR/Cas9 KO Plasmid (h) and FBXW2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FBXW2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FBXW2 HDR Plasmid (h) and FBXW2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FBXW2 homology arms to support homology-directed repair at defined FBXW2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.