FBXO4 Antibody (D-9): sc-376372
- FBXO4 Antibody (D-9) is a mouse monoclonal IgG1 κ FBXO4 antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 59-358 mapping within an internal region of FBXO4 of human origin
- FBXO4 Antibody (D-9) is recommended for detection of FBXO4 of human origin by WB, IP, IF and ELISA
- Anti-FBXO4 Antibody (D-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for FBXO4 Antibody (D-9) for WB applications. This reagent is now offered in a bundle with FBXO4 Antibody (D-9) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
FBXO4 Antibody (D-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects FBXO4 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). FBXO4 (D-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FBXO4 plays a crucial role as a substrate recognition component of the SCF (Skp1-CUL-1-F-box protein)-type E3 ubiquitin ligase complex, which is essential for the ubiquitination process that regulates protein degradation. This regulation is vital for maintaining cellular homeostasis, as FBXO4 controls the levels of key proteins involved in various cellular processes, including the cell cycle, immune response, and signaling pathways. FBXO4 specifically recognizes and binds to phosphorylated target proteins, facilitating their ubiquitination and subsequent degradation by the proteasome. FBXO4 directly interacts with Skp1 p19 and CUL-1 within the SCF-type E3 complex and promotes the ubiquitination of TRF1, a protein involved in telomere maintenance. FBXO4 is expressed in two isoforms due to alternative splicing, which may contribute to its functional diversity in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
FBXO4 Antibody (D-9) References:
- Identification of a family of human F-box proteins. | Cenciarelli, C., et al. 1999. Curr Biol. 9: 1177-9. PMID: 10531035
- A family of mammalian F-box proteins. | Winston, JT., et al. 1999. Curr Biol. 9: 1180-2. PMID: 10531037
- Five human genes encoding F-box proteins: chromosome mapping and analysis in human tumors. | Chiaur, DS., et al. 2000. Cytogenet Cell Genet. 88: 255-8. PMID: 10828603
- Systematic analysis and nomenclature of mammalian F-box proteins. | Jin, J., et al. 2004. Genes Dev. 18: 2573-80. PMID: 15520277
- The F-box protein FBX4 targets PIN2/TRF1 for ubiquitin-mediated degradation and regulates telomere maintenance. | Lee, TH., et al. 2006. J Biol Chem. 281: 759-68. PMID: 16275645
- The ETS protein MEF is regulated by phosphorylation-dependent proteolysis via the protein-ubiquitin ligase SCFSkp2. | Liu, Y., et al. 2006. Mol Cell Biol. 26: 3114-23. PMID: 16581786
- Phosphorylation-dependent ubiquitination of cyclin D1 by the SCF(FBX4-alphaB crystallin) complex. | Lin, DI., et al. 2006. Mol Cell. 24: 355-66. PMID: 17081987
- Mutations in Fbx4 inhibit dimerization of the SCF(Fbx4) ligase and contribute to cyclin D1 overexpression in human cancer. | Barbash, O., et al. 2008. Cancer Cell. 14: 68-78. PMID: 18598945
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FBXO4 Antibody (D-9) | sc-376372 | 200 µg/ml | $316.00 | |||
FBXO4 Antibody (D-9): m-IgGκ BP-HRP Bundle | sc-522931 | 200 µg Ab, 40 µg BP | $354.00 | |||
FBXO4 Antibody (D-9) AC | sc-376372 AC | 500 µg/ml, 25% agarose | $416.00 | |||
FBXO4 Antibody (D-9) HRP | sc-376372 HRP | 200 µg/ml | $316.00 | |||
FBXO4 Antibody (D-9) FITC | sc-376372 FITC | 200 µg/ml | $330.00 | |||
FBXO4 Antibody (D-9) PE | sc-376372 PE | 200 µg/ml | $343.00 | |||
FBXO4 Antibody (D-9) Alexa Fluor® 488 | sc-376372 AF488 | 200 µg/ml | $357.00 | |||
FBXO4 Antibody (D-9) Alexa Fluor® 546 | sc-376372 AF546 | 200 µg/ml | $357.00 | |||
FBXO4 Antibody (D-9) Alexa Fluor® 594 | sc-376372 AF594 | 200 µg/ml | $357.00 | |||
FBXO4 Antibody (D-9) Alexa Fluor® 647 | sc-376372 AF647 | 200 µg/ml | $357.00 | |||
FBXO4 Antibody (D-9) Alexa Fluor® 680 | sc-376372 AF680 | 200 µg/ml | $357.00 | |||
FBXO4 Antibody (D-9) Alexa Fluor® 790 | sc-376372 AF790 | 200 µg/ml | $357.00 |