FBXO30 Antibody (E-10): sc-514862

FBXO30 Antibody (E-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects FBXO30 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). FBXO30 Antibody (E-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FBXO30, also known as F-box only protein 30, is a 745 amino acid protein characterized by a 40-amino acid F-box domain and a TRAF-type zinc finger, positioning FBXO30 as a vital member of the F-box protein family. F-box proteins play a crucial role in the SCF (Skp1-CUL-1-F-box protein) E3 ubiquitin ligase complex, which is essential for substrate recognition and recruitment for ubiquitination. FBXO30 is particularly important in regulating various cellular processes, including cell cycle, immune response, and signaling pathways, by targeting specific proteins for degradation via the proteasome. FBXO30 selectively recognizes and binds to phosphorylated proteins, facilitating ubiquitination and subsequent degradation, which underscores FBXO30′s significance in maintaining cellular homeostasis and regulating protein levels in response to cellular signals.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

FBXO30 Antibody (E-10) References:

1. Identification of a family of human F-box proteins.  |  Cenciarelli, C., et al. 1999. Curr Biol. 9: 1177-9. PMID: 10531035
2. A family of mammalian F-box proteins.  |  Winston, JT., et al. 1999. Curr Biol. 9: 1180-2. PMID: 10531037
3. The F-box: a new motif for ubiquitin dependent proteolysis in cell cycle regulation and signal transduction.  |  Craig, KL. and Tyers, M. 1999. Prog Biophys Mol Biol. 72: 299-328. PMID: 10581972
4. cDNA cloning and expression analysis of new members of the mammalian F-box protein family.  |  Ilyin, GP., et al. 2000. Genomics. 67: 40-7. PMID: 10945468
5. Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex.  |  Schulman, BA., et al. 2000. Nature. 408: 381-6. PMID: 11099048
6. A new subfamily of structurally related human F-box proteins.  |  Ilyin, GP., et al. 2002. Gene. 296: 11-20. PMID: 12383498
7. [Study on the fusion expression of FBXO30: a novel member of F-box protein family].  |  Li, ZH., et al. 2001. Hunan Yi Ke Da Xue Xue Bao. 26: 495-8. PMID: 12536519
8. Fbxo30 Regulates Mammopoiesis by Targeting the Bipolar Mitotic Kinesin Eg5.  |  Liu, Y., et al. 2016. Cell Rep. 15: 1111-1122. PMID: 27117404
9. Fbxo30 regulates chromosome segregation of oocyte meiosis.  |  Jin, Y., et al. 2019. Cell Mol Life Sci. 76: 2217-2229. PMID: 30980108
10. F-box protein FBXO30 mediates retinoic acid receptor γ ubiquitination and regulates BMP signaling in neural tube defects.  |  Cheng, X., et al. 2019. Cell Death Dis. 10: 551. PMID: 31320612
11. FBXO30 functions as a tumor suppressor and an E3 ubiquitin ligase for hZIP1‑mediated HIF‑1α degradation in renal cell carcinoma.  |  Yuan, Y., et al. 2023. Int J Oncol. 62: PMID: 36799168
12. The SCFbeta-TRCP-ubiquitin ligase complex associates specifically with phosphorylated destruction motifs in IkappaBalpha and beta-catenin and stimulates IkappaBalpha ubiquitination in vitro.  |  Winston, JT., et al. 1999. Genes Dev. 13: 270-83. PMID: 9990852

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FBXO30 Antibody (E-10) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-FBXO30 Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FBXO30 Antibody (E-10) Specifications

Alternate Product Name: FBXO30

Product Clone Name: E-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 411-534

Epitope Target: FBXO30

Epitope Species: human

Product Reactivity/Detection: FBXO30

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for FBXO30 Antibody (E-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin