FBL5 Antibody (G-11): sc-390102
- FBL5 Antibody (G-11) is a mouse monoclonal IgG1 κ FBL5 antibody provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of FBL5 of human origin
- FBL5 Antibody (G-11) is recommended for detection of FBL5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-FBL5 Antibody (G-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for FBL5 Antibody (G-11) for WB and IHC(P) applications. These reagents are now offered in bundles with FBL5 Antibody (G-11) (see ordering information below).
FBL5 Antibody (G-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects FBL5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). FBL5 (G-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FBL5 protein, consisting of 691 amino acids and encoded by the human gene FBXL5, plays a crucial role in protein degradation regulation through involvement in the SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex. This complex is essential for substrate recognition and recruitment, targeting proteins such as cyclins and IκB-α for ubiquitination and subsequent degradation by the proteasome. FBL5 contains a 40 amino acid F-box region and four leucine-rich repeats, which are vital for mediating protein interactions and facilitating the ubiquitination process. FBL5 is predominantly localized in the cytoplasm near the nucleus and is ubiquitously expressed, indicating importance in various cellular processes, including cell cycle regulation and immune response. Through recognition and binding to phosphorylated proteins, FBL5 promotes ubiquitination, thereby influencing stability and turnover of key regulatory proteins within the cell.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
FBL5 Antibody (G-11) References:
- Identification of a family of human F-box proteins. | Cenciarelli, C., et al. 1999. Curr Biol. 9: 1177-9. PMID: 10531035
- A family of mammalian F-box proteins. | Winston, JT., et al. 1999. Curr Biol. 9: 1180-2. PMID: 10531037
- The F-box: a new motif for ubiquitin dependent proteolysis in cell cycle regulation and signal transduction. | Craig, KL. and Tyers, M. 1999. Prog Biophys Mol Biol. 72: 299-328. PMID: 10581972
- cDNA cloning and expression analysis of new members of the mammalian F-box protein family. | Ilyin, GP., et al. 2000. Genomics. 67: 40-7. PMID: 10945468
- Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex. | Schulman, BA., et al. 2000. Nature. 408: 381-6. PMID: 11099048
- A new subfamily of structurally related human F-box proteins. | Ilyin, GP., et al. 2002. Gene. 296: 11-20. PMID: 12383498
- The SCFbeta-TRCP-ubiquitin ligase complex associates specifically with phosphorylated destruction motifs in IkappaBalpha and beta-catenin and stimulates IkappaBalpha ubiquitination in vitro. | Winston, JT., et al. 1999. Genes Dev. 13: 270-83. PMID: 9990852
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FBL5 Antibody (G-11) | sc-390102 | 200 µg/ml | $316.00 | |||
FBL5 Antibody (G-11): m-IgG Fc BP-HRP Bundle | sc-526121 | 200 µg Ab; 10 µg BP | $354.00 | |||
FBL5 Antibody (G-11): m-IgG1 BP-HRP Bundle | sc-550726 | 200 µg Ab; 20 µg BP | $354.00 | |||
FBL5 Antibody (G-11) AC | sc-390102 AC | 500 µg/ml, 25% agarose | $416.00 | |||
FBL5 Antibody (G-11) HRP | sc-390102 HRP | 200 µg/ml | $316.00 | |||
FBL5 Antibody (G-11) FITC | sc-390102 FITC | 200 µg/ml | $330.00 | |||
FBL5 Antibody (G-11) PE | sc-390102 PE | 200 µg/ml | $343.00 | |||
FBL5 Antibody (G-11) Alexa Fluor® 488 | sc-390102 AF488 | 200 µg/ml | $357.00 | |||
FBL5 Antibody (G-11) Alexa Fluor® 546 | sc-390102 AF546 | 200 µg/ml | $357.00 | |||
FBL5 Antibody (G-11) Alexa Fluor® 594 | sc-390102 AF594 | 200 µg/ml | $357.00 | |||
FBL5 Antibody (G-11) Alexa Fluor® 647 | sc-390102 AF647 | 200 µg/ml | $357.00 | |||
FBL5 Antibody (G-11) Alexa Fluor® 680 | sc-390102 AF680 | 200 µg/ml | $357.00 | |||
FBL5 Antibody (G-11) Alexa Fluor® 790 | sc-390102 AF790 | 200 µg/ml | $357.00 |