FBL10 Double Nickase Plasmid (h): sc-403232-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FBL10 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FBL10 Double Nickase Plasmid (h) and FBL10 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KDM2B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FBL10 Antibody (5G1): sc-293279
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FBL10 Double Nickase Plasmid (h) | sc-403232-NIC | 20 µg | $410.00 | |||
FBL10 Double Nickase Plasmid (h2) | sc-403232-NIC-2 | 20 µg | $410.00 |
Human KDM2B encodes the FBL10 protein, a JmjC-domain histone demethylase and chromatin-associated factor that links CpG island recognition to transcriptional regulation. FBL10 participates in epigenetic control of gene expression through histone lysine demethylation and coordination with Polycomb-associated mechanisms, influencing chromatin accessibility and developmental gene programs. By modulating pathways involved in cell-cycle progression, differentiation, and maintenance of cellular identity, KDM2B/FBL10 activity helps shape lineage-specific transcriptional states. Dysregulation of KDM2B has been reported in multiple disease-relevant contexts, including oncogenic transcriptional rewiring and altered stem-like phenotypes, making it a useful node for mechanistic studies of epigenetic regulation.
FBL10 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KDM2B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KDM2B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KDM2B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KDM2B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.