Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FATE1 Double Nickase Plasmid (h): sc-413809-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FATE1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FATE1 Double Nickase Plasmid (h) and FATE1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FATE1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FATE1 Antibody (B5-9): sc-101220
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FATE1 Double Nickase Plasmid (h) | sc-413809-NIC | 20 µg | $410.00 |
FATE1 (fetal and adult testis-expressed 1) encodes a mitochondria-associated protein enriched in testis and frequently upregulated in multiple tumor contexts, where it can influence cellular stress tolerance. Reported to localize at mitochondria–endoplasmic reticulum contact sites, FATE1 has been linked to regulation of calcium transfer, mitochondrial membrane dynamics, and apoptotic signaling pathways. By modulating organelle crosstalk and mitochondrial susceptibility to stress, FATE1 can affect proliferation and survival programs that are relevant to cancer biology. These properties make FATE1 a useful target for studying mitochondrial regulation, ER–mitochondria tethering, and stress-response networks in human cells.
FATE1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FATE1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FATE1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FATE1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FATE1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.