Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FAST-1/2 CRISPR/Cas9 KO Plasmid (m): sc-420290
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FAST-1/2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FAST-1/2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FAST-1/2 CRISPR/Cas9 KO Plasmid (m) | sc-420290 | 20 µg | $397.00 |
Overview
Foxh1 (FAST-1/2) is a forkhead box transcription factor that functions as a key nuclear effector of Activin/Nodal–TGF-β signaling in mouse development. By partnering with SMAD2/3–SMAD4 complexes, it regulates transcriptional programs controlling mesendoderm induction, left–right axis specification, and early patterning decisions. Foxh1-dependent gene networks intersect with pathways governing epithelial–mesenchymal transitions and lineage commitment, making it a widely used node for dissecting signal-dependent transcriptional control. Perturbation of this axis is relevant to developmental malformations and to mechanistic studies of TGF-β–driven transcriptional reprogramming in disease-associated contexts.
FAST-1/2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Foxh1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Foxh1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Foxh1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FAST-1/2 protein expression.
This CRISPR knockout system enables efficient generation of Foxh1-deficient cell models for investigation of FAST-1/2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Foxh1 exon(s) critical for FAST-1/2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Foxh1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FAST-1/2 CRISPR/Cas9 KO Plasmid (m) and FAST-1/2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Foxh1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FAST-1/2 HDR Plasmid (m) and FAST-1/2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Foxh1 homology arms to support homology-directed repair at defined Foxh1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.