FAPα Double Nickase Plasmid (h): sc-401511-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FAPα Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FAPα Double Nickase Plasmid (h) and FAPα Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FAP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FAPα Antibody (SS-13): sc-100528
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FAPα Double Nickase Plasmid (h) | sc-401511-NIC | 20 µg | $410.00 | |||
FAPα Double Nickase Plasmid (h2) | sc-401511-NIC-2 | 20 µg | $410.00 |
Fibroblast activation protein alpha (FAPα) is a type II transmembrane serine protease of the S9B dipeptidyl peptidase family with both dipeptidyl peptidase and endopeptidase activity. It is prominently expressed by activated fibroblasts and stromal populations and contributes to extracellular matrix remodeling through cleavage of proline-rich substrates, influencing cell–matrix interactions, adhesion, and migratory behavior. FAPα activity intersects with stromal signaling programs that regulate tissue remodeling and inflammatory microenvironments, including pathways linked to fibroblast activation states. Altered FAP expression is frequently associated with fibrotic remodeling and tumor-associated stroma, making it a useful marker and functional node for studying microenvironmental regulation in human disease contexts.
FAPα Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FAP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FAP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FAP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FAP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.