FANCF Antibody (D-2): sc-271952
- FANCF Antibody (D-2) is a mouse monoclonal IgG1 κ FANCF antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 41-350 of FANCF of human origin
- FANCF Antibody (D-2) is recommended for detection of FANCF of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-FANCF Antibody (D-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for FANCF Antibody (D-2). This work is in progress.
FANCF Antibody (D-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects FANCF protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-FANCF antibody (D-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FANCF protein plays a crucial role in the Fanconi anemia (FA) pathway, which is essential for the repair of DNA interstrand cross-links, thereby maintaining genomic stability. Mutations in FANCF gene can lead to Fanconi anemia, a genetic disorder characterized by bone marrow failure, developmental abnormalities, and increased cancer risk due to chromosomal instability. FANCF protein is involved in the activation of FANCD2, a key player in the FA pathway, and stabilizes other subunits of the FA complex. Notably, phosphorylation of FANCF and other FA proteins is critical for their function, as this process regulates activity and interactions within the cellular environment. Human FANCF gene is located on chromosome 11p15 and encodes a nuclear protein that shares homology with the prokaryotic RNA-binding protein ROM, highlighting potential roles in RNA processing and cellular responses to DNA damage.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
FANCF Antibody (D-2) References:
- The Fanconi anemia protein FANCF forms a nuclear complex with FANCA, FANCC and FANCG. | de Winter, JP., et al. 2000. Hum Mol Genet. 9: 2665-74. PMID: 11063725
- A cytoplasmic serine protein kinase binds and may regulate the Fanconi anemia protein FANCA. | Yagasaki, H., et al. 2001. Blood. 98: 3650-7. PMID: 11739169
- Function of the Fanconi anemia pathway in Fanconi anemia complementation group F and D1 cells. | Siddique, MA., et al. 2001. Exp Hematol. 29: 1448-55. PMID: 11750104
- The Chinese hamster FANCG/XRCC9 mutant NM3 fails to express the monoubiquitinated form of the FANCD2 protein, is hypersensitive to a range of DNA damaging agents and exhibits a normal level of spontaneous sister chromatid exchange. | Wilson, JB., et al. 2001. Carcinogenesis. 22: 1939-46. PMID: 11751423
- Phosphorylation of Fanconi anemia protein, FANCA, is regulated by Akt kinase. | Otsuki, T., et al. 2002. Biochem Biophys Res Commun. 291: 628-34. PMID: 11855836
- Molecular pathogenesis of fanconi anemia. | Taniguchi, T. and Dandrea, AD. 2002. Int J Hematol. 75: 123-8. PMID: 11939257
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FANCF Antibody (D-2) | sc-271952 | 200 µg/ml | $322.00 | |||
FANCF Antibody (D-2) AC | sc-271952 AC | 500 µg/ml, 25% agarose | $424.00 | |||
FANCF Antibody (D-2) HRP | sc-271952 HRP | 200 µg/ml | $322.00 | |||
FANCF Antibody (D-2) FITC | sc-271952 FITC | 200 µg/ml | $336.00 | |||
FANCF Antibody (D-2) PE | sc-271952 PE | 200 µg/ml | $349.00 | |||
FANCF Antibody (D-2) Alexa Fluor® 488 | sc-271952 AF488 | 200 µg/ml | $364.00 | |||
FANCF Antibody (D-2) Alexa Fluor® 546 | sc-271952 AF546 | 200 µg/ml | $364.00 | |||
FANCF Antibody (D-2) Alexa Fluor® 594 | sc-271952 AF594 | 200 µg/ml | $364.00 | |||
FANCF Antibody (D-2) Alexa Fluor® 647 | sc-271952 AF647 | 200 µg/ml | $364.00 | |||
FANCF Antibody (D-2) Alexa Fluor® 680 | sc-271952 AF680 | 200 µg/ml | $364.00 | |||
FANCF Antibody (D-2) Alexa Fluor® 790 | sc-271952 AF790 | 200 µg/ml | $364.00 |