FAM188B CRISPR/Cas9 KO Plasmid (h): sc-413571
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FAM188B CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FAM188B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FAM188B CRISPR/Cas9 KO Plasmid (h) | sc-413571 | 20 µg | $397.00 |
Overview
FAM188B encodes a poorly characterized human protein implicated in the regulation of protein homeostasis and signaling through putative associations with ubiquitin-dependent turnover and proteostasis networks. Emerging evidence links FAM188B to cellular proliferation and stress-adaptive responses, suggesting relevance to pathways that coordinate cell-cycle progression, survival signaling, and adaptation to proteotoxic conditions. Altered expression patterns have been reported across multiple tumor types, supporting investigation of FAM188B as a modulator of oncogenic phenotypes and context-dependent vulnerabilities. Despite limited mechanistic definition, FAM188B is a useful target for dissecting how proteome regulation interfaces with growth control and cellular fitness.
FAM188B CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FAM188B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FAM188B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FAM188B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FAM188B protein expression.
This CRISPR knockout system enables efficient generation of FAM188B-deficient cell models for investigation of FAM188B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FAM188B exon(s) critical for FAM188B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FAM188B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FAM188B CRISPR/Cas9 KO Plasmid (h) and FAM188B CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FAM188B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FAM188B HDR Plasmid (h) and FAM188B HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FAM188B homology arms to support homology-directed repair at defined FAM188B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.