FAM127B CRISPR/Cas9 KO Plasmid (h): sc-406247
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FAM127B CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FAM127B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FAM127B CRISPR/Cas9 KO Plasmid (h) | sc-406247 | 20 µg | $397.00 |
Overview
FAM127B (family with sequence similarity 127 member B) encodes a poorly characterized human protein with limited functional annotation, making it of interest for systematic genetic interrogation. Available evidence suggests roles in cellular homeostasis and gene regulatory processes, with potential connections to signaling and transcriptional programs that influence proliferation and stress responses. As with other understudied FAM family members, altered expression or copy number has been reported in subsets of human disease datasets, supporting investigation in cancer and other complex phenotypes. Dissecting FAM127B function can help clarify its contribution to pathway wiring, cell-state maintenance, and context-dependent vulnerabilities.
FAM127B CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FAM127B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FAM127B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FAM127B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FAM127B protein expression.
This CRISPR knockout system enables efficient generation of FAM127B-deficient cell models for investigation of FAM127B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FAM127B exon(s) critical for FAM127B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FAM127B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FAM127B CRISPR/Cas9 KO Plasmid (h) and FAM127B CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FAM127B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FAM127B HDR Plasmid (h) and FAM127B HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FAM127B homology arms to support homology-directed repair at defined FAM127B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.