Factor VIII light chain Antibody (RFFVIII C/5): sc-59512

Factor VIII light chain Antibody (RFFVIII C/5) is a mouse monoclonal IgG1 kappa light chain antibody designed to target the light chain of human Factor VIII protein, also known as anti-hemophilic factor or von Willebrand factor-binding protein. Factor VIII is a vital glycoprotein involved in the blood coagulation cascade, consisting of both heavy and light chains that work together to activate Factor X to Factor Xa, ultimately leading to stable blood clot formation. The light chain of Factor VIII mediates binding to von Willebrand factor (VWF), which stabilizes Factor VIII in the bloodstream and ensures proper function. Factor VIII (RFFVIII C/5) monoclonal antibody effectively detects the full-length 360 kDa Factor VIII protein as well as the 80 kDa doublet bands in both human and porcine samples through western blotting (WB), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP) assays. Understanding the structural integrity of Factor VIII light chain is crucial for studying hemophilia A, a severe hereditary bleeding disorder caused by mutations that disrupt Factor VIII′s function or stability. Anti-Factor VIII antibody (RFFVIII C/5) facilitates research into molecular mechanisms of coagulation and development of targeted therapeutic interventions. Factor VIII (RFFVIII C/5) monoclonal antibody′s compatibility with multiple assay types makes this reagent an indispensable tool for laboratories focused on hemostasis, blood coagulation, and related biomedical research. Factor VIII (RFFVIII C/5) antibody provides researchers with a reliable means to investigate structural and functional aspects of Factor VIII, contributing to advancements in understanding and treating coagulation disorders.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Factor VIII light chain Antibody (RFFVIII C/5) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Factor VIII light chain Antibody (RFFVIII C/5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Factor VIII light chain Antibody (RFFVIII C/5) Specifications

Alternate Product Name: Factor VIII light chain

Product Clone Name: RFFVIII C/5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Factor VIII light chain

Epitope Species: human

Product Reactivity/Detection: the 360 kDa band of Factor VIII as well as the 80 kDa doublet bands

Species Reactivity/Detection: human, porcine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Factor VIII light chain Antibody (RFFVIII C/5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Factor VIII antibody is Factor VIII Antibody (R8B12): sc-73597.