Factor VIII Antibody (GMA-8011): sc-65950

Factor VIII Antibody (GMA-8011) is a mouse monoclonal IgG2a antibody engineered to target the C1 domain of B domain-deleted recombinant human Factor VIII, also known as anti-hemophilic factor or von Willebrand factor-binding protein. Factor VIII is a pivotal glycoprotein cofactor in the intrinsic pathway of the blood coagulation cascade, essential for the activation of Factor X to Factor Xa, which ultimately leads to the formation of stable blood clots. Anti-Factor VIII antibody (GMA-8011) effectively detects human Factor VIII through enzyme-linked immunosorbent assay (ELISA) assays, ensuring reliable performance for both research and diagnostic applications. Factor VIII is primarily synthesized in the liver, where Factor VIII is localized within the endoplasmic reticulum, a crucial cellular compartment responsible for proper protein folding and assembly. Within the endoplasmic reticulum, Factor VIII interacts with chaperone proteins such as Calreticulin and Calnexin, which are essential for correct folding and stability, ensuring Factor VIII is properly processed and functional before being secreted into the bloodstream. The precise cellular localization of Factor VIII is vital for maturation and subsequent binding to von Willebrand factor (VWF), which stabilizes Factor VIII in circulation and protects Factor VIII from premature degradation. By targeting the C1 domain, Factor VIII (GMA-8011) antibody facilitates detailed studies on the intracellular processing and trafficking of Factor VIII, providing valuable insights into the molecular mechanisms underlying hemophilia A, a severe hereditary bleeding disorder caused by deficiencies or dysfunctions of Factor VIII. Additionally, the specificity of Factor VIII monoclonal antibody (GMA-8011) for the C1 domain minimizes cross-reactivity, enhancing utility in various experimental settings. Factor VIII (GMA-8011) monoclonal antibody supports advanced research into coagulation processes, the development of targeted therapeutic interventions, and the improvement of diagnostic assays for Factor VIII-related disorders. Overall, anti-Factor VIII antibody (GMA-8011) is an indispensable tool for scientists and healthcare professionals dedicated to advancing the understanding and treatment of coagulation disorders.

The preferred Factor VIII antibody is Factor VIII Antibody (R8B12): sc-73597.