Factor VIII Antibody (102): sc-59510

Factor VIII Antibody (102) is a mouse monoclonal IgG1 antibody engineered to recognize the full-length native human Factor VIII protein, also known as anti-hemophilic factor or von Willebrand factor-binding protein. Factor VIII is a critical glycoprotein cofactor located primarily in the endoplasmic reticulum of liver cells, where it plays an essential role in the intrinsic pathway of blood coagulation by facilitating the activation of Factor X to Factor Xa, ultimately leading to the formation of stable blood clots. Anti-Factor VIII antibody (102) effectively detects the full-length 360 kDa Factor VIII protein in human samples through western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). The precise cellular localization of Factor VIII within the endoplasmic reticulum is crucial for proper folding and interaction with chaperone proteins such as Calreticulin and Calnexin, which are necessary for stability and functionality. Understanding the intracellular dynamics and function of Factor VIII is vital for studying hemophilia A, a severe hereditary bleeding disorder caused by insufficient or dysfunctional Factor VIII. Factor VIII (102) monoclonal antibody enables researchers to investigate the synthesis, processing, and secretion of Factor VIII, providing valuable insights into the molecular mechanisms underlying coagulation disorders. By facilitating accurate detection and quantification of Factor VIII, Factor VIII (102) antibody supports both clinical diagnostics and the development of therapeutic strategies aimed at enhancing Factor VIII function and stability.

The preferred Factor VIII antibody is Factor VIII Antibody (R8B12): sc-73597.