F-Spondin Double Nickase Plasmid (h): sc-404101-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- F-Spondin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- F-Spondin Double Nickase Plasmid (h) and F-Spondin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SPON1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: F-Spondin Antibody (B-3): sc-390182
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
F-Spondin Double Nickase Plasmid (h) | sc-404101-NIC | 20 µg | $410.00 | |||
F-Spondin Double Nickase Plasmid (h2) | sc-404101-NIC-2 | 20 µg | $410.00 |
SPON1 encodes F-spondin, a secreted extracellular matrix glycoprotein that modulates cell–matrix adhesion and axon guidance during neural development and tissue remodeling. Through its spondin and thrombospondin type 1 repeat domains, F-spondin influences neurite outgrowth, synaptic connectivity, and interactions with heparan sulfate proteoglycans and integrin-associated signaling. SPON1 has been studied in pathways linked to neuroinflammation, extracellular matrix organization, and vascular or stromal remodeling, with reported associations to neurodegenerative and neurodevelopmental phenotypes. These features make SPON1 a useful target for probing how ECM cues shape neuronal wiring and cell migration programs in human model systems.
F-Spondin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SPON1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SPON1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SPON1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SPON1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.