EYA2 Double Nickase Plasmid (h): sc-403115-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EYA2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EYA2 Double Nickase Plasmid (h) and EYA2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EYA2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EYA2 Antibody (F-18): sc-100325
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EYA2 Double Nickase Plasmid (h) | sc-403115-NIC | 20 µg | $410.00 | |||
EYA2 Double Nickase Plasmid (h2) | sc-403115-NIC-2 | 20 µg | $410.00 |
EYA2 encodes Eyes Absent homolog 2, a multifunctional transcriptional coactivator and haloacid dehalogenase–like phosphatase that regulates developmental and homeostatic gene expression programs. Through interactions with SIX family transcription factors and modulation of tyrosine phosphorylation states, EYA2 contributes to cell fate decisions, proliferation, migration, and DNA damage–associated signaling. EYA2-linked networks intersect with organogenesis pathways and transcriptional control circuits that shape epithelial and mesenchymal phenotypes. Dysregulated EYA2 expression or activity has been reported in multiple disease-associated contexts, making it a useful target for dissecting pathway rewiring and genotype–phenotype relationships in human cell models.
EYA2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EYA2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EYA2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EYA2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EYA2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.