EYA2 Antibody (F-18) recommended for detection of EYA2 of human origin by WB, IP, IF and ELISA

EYA2 Antibody (F-18): sc-100325

EYA2 Antibody (F-18) is rated 4.0 out of 5 by 2.
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Datasheets

    • Anti-EYA2 Antibody (F-18) is a mouse monoclonal IgG2a (kappa light chain) EYA2 antibody provided at 100 µg/ml
    • raised against recombinant EYA2 of human origin
    • recommended for detection of EYA2 of human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of EYA2 (F-18): sc-100325.

I am using EYA2 (F-18): sc-100325 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Robust immunofluorescence cytoplasmic staining Robust immunofluorescence cytoplasmic staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-05-02
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