EYA2 Antibody (F-18): sc-100325

EYA2 Antibody (F-18) is a mouse monoclonal IgG2a kappa light chain antibody that detects EYA2 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-EYA2 antibody (F-18) is available as a non-conjugated format. EYA2, encoded by a gene located on chromosome 20q13.1, is a member of the eyes absent family, which consists of four related proteins. EYA2 plays a crucial role in developmental processes, particularly in myogenesis, where Pax3 induces EYA2 in a signaling cascade essential for muscle development. EYA2 features a highly conserved 271 amino acid domain at its carboxy-terminal, which is critical for function as a transcriptional activator, while the amino-terminal region is rich in proline, serine, and threonine residues, contributing to diverse regulatory roles. EYA2 is predominantly expressed in the cytoplasm during late developmental stages in various tissues, including extensor tendons, ligaments, cranial placodes, branchial arches, the central nervous system, and the developing eye. Six proteins facilitate EYA2′s translocation to the nucleus through homeodomain binding, allowing function in connective tissue patterning. Notably, EYA2′s carboxy-terminal interacts with Gαz and Gαi2 proteins, which can inhibit nuclear translocation, highlighting the intricate regulatory mechanisms governing EYA2′s activity in cellular processes.

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EYA2 Antibody (F-18) References:

1. Eya1-deficient mice lack ears and kidneys and show abnormal apoptosis of organ primordia.  |  Xu, PX., et al. 1999. Nat Genet. 23: 113-7. PMID: 10471511
2. Cooperation of six and eya in activation of their target genes through nuclear translocation of Eya.  |  Ohto, H., et al. 1999. Mol Cell Biol. 19: 6815-24. PMID: 10490620
3. The alpha subunits of Gz and Gi interact with the eyes absent transcription cofactor Eya2, preventing its interaction with the six class of homeodomain-containing proteins.  |  Fan, X., et al. 2000. J Biol Chem. 275: 32129-34. PMID: 10906137
4. Pax3 is essential for skeletal myogenesis and the expression of Six1 and Eya2.  |  Ridgeway, AG. and Skerjanc, IS. 2001. J Biol Chem. 276: 19033-9. PMID: 11262400
5. Mouse Eya homologues of the Drosophila eyes absent gene require Pax6 for expression in lens and nasal placode.  |  Xu, PX., et al. 1997. Development. 124: 219-31. PMID: 9006082
6. A human homologue of the Drosophila eyes absent gene underlies branchio-oto-renal (BOR) syndrome and identifies a novel gene family.  |  Abdelhak, S., et al. 1997. Nat Genet. 15: 157-64. PMID: 9020840
7. Eyes absent: a gene family found in several metazoan phyla.  |  Duncan, MK., et al. 1997. Mamm Genome. 8: 479-85. PMID: 9195991
8. Mouse Eya genes are expressed during limb tendon development and encode a transcriptional activation function.  |  Xu, PX., et al. 1997. Proc Natl Acad Sci U S A. 94: 11974-9. PMID: 9342347

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EYA2 Antibody (F-18) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-EYA2 Antibody (F-18) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EYA2 Antibody (F-18) Specifications

Alternate Product Name: EYA2

Product Clone Name: F-18

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 164-272

Epitope Target: EYA2

Epitope Species: human

Product Reactivity/Detection: EYA2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for EYA2 Antibody (F-18): N/A